| Tag | Content |
|---|
| CPLM ID | CPLM-014279 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Importin subunit alpha-5 |
Protein Synonyms/Alias | Importin alpha-S1; Karyopherin subunit alpha-1; Nucleoprotein interactor 1; NPI-1; RAG cohort protein 2; SRP1-beta |
Gene Name | Kpna1 |
Gene Synonyms/Alias | Rch2 |
Created Date | July 27, 2013 |
Organism | Mus musculus (Mouse) |
NCBI Taxa ID | 10090 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 136 | ARFVEFLKRKENCTL | ubiquitination | [1] | | 398 | TAEFRTRKEAAWAIT | ubiquitination | [1] |
|
Reference | [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C. Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [ PMID: 22790023] |
Functional Description | Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. |
Sequence Annotation | DOMAIN 1 57 IBB.
REPEAT 77 117 ARM 1; truncated.
REPEAT 118 161 ARM 2.
REPEAT 162 206 ARM 3.
REPEAT 207 245 ARM 4.
REPEAT 246 290 ARM 5.
REPEAT 291 330 ARM 6.
REPEAT 331 372 ARM 7.
REPEAT 373 412 ARM 8.
REPEAT 413 457 ARM 9.
REPEAT 460 504 ARM 10; atypical.
REGION 149 241 NLS binding site (major) (By similarity).
REGION 245 437 Binding to RAG1 (By similarity).
REGION 318 406 NLS binding site (minor) (By similarity).
MOTIF 42 51 Nuclear localization signal (By
MOD_RES 3 3 Phosphothreonine. |
Keyword | Complete proteome; Cytoplasm; Nucleus; Phosphoprotein; Protein transport; Reference proteome; Repeat; Transport; Ubl conjugation. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 538 AA |
Protein Sequence | MSTPGKENFR LKSYKNKSLN PDEMRRRREE EGLQLRKQKR EEQLFKRRNV ATAEEETEEE 60
VMSDGGFHEA QINNMEMAPG GVITSDMTDM IFSNSPEQQL SATQKFRKLL SKEPNPPIDE 120
VINTPGVVAR FVEFLKRKEN CTLQFESAWV LTNIASGNSL QTRNVIQAGA VPIFIELLSS 180
EFEDVQEQAV WALGNIAGDS TMCRDYVLNC NILPPLLQLF SKQNRLTMTR NAVWALSNLC 240
RGKSPPPEFA KVSPCLNVLS WLLFVSDTDV LADACWALSY LSDGPNDKIQ AVIDAGVCRR 300
LVELLMHNDY KVVSPALRAV GNIVTGDDIQ TQVILNCSAL QSLLHLLSSP KESIKKEACW 360
TISNITAGNR AQIQTVIDAN MFPALISILQ TAEFRTRKEA AWAITNATSG GSAEQIKYLV 420
ELGCIKPLCD LLTVMDAKIV QVALNGLENI LRLGEQEAKR NGSGINPYCA LIEEAYGLDK 480
IEFLQSHENQ EIYQKAFDLI EHYFGTEDED SSIAPQVDLS QQQYIFQQCE APMEGFQL 538 |
Gene Ontology | GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. GO:0030425; C:dendrite; ISS:UniProtKB. GO:0005634; C:nucleus; ISS:UniProtKB. GO:0008565; F:protein transporter activity; IEA:InterPro. GO:0042307; P:positive regulation of protein import into nucleus; ISS:UniProtKB. GO:0006606; P:protein import into nucleus; IEA:InterPro. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |