CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023263
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NSFL1 cofactor p47 
Protein Synonyms/Alias
 UBX domain-containing protein 2C; p97 cofactor p47 
Gene Name
 NSFL1C 
Gene Synonyms/Alias
 UBXN2C 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
114QIVGPPRKKSPNELVubiquitination[1, 2, 3]
115IVGPPRKKSPNELVDubiquitination[1, 2, 3, 4]
126ELVDDLFKGAKEHGAubiquitination[1, 2, 3, 4, 5]
129DDLFKGAKEHGAVAVubiquitination[1, 2, 4, 5]
141VAVERVTKSPGETSKubiquitination[5]
148KSPGETSKPRPFAGGubiquitination[1, 2, 3, 4, 5]
174SAYVAGEKRQHSSQDubiquitination[1, 2, 3, 4, 5]
190HVVLKLWKSGFSLDNubiquitination[1, 2, 3, 4, 5]
247HRDEDFVKPKGAFKAubiquitination[2, 5]
253VKPKGAFKAFTGEGQubiquitination[1, 2]
261AFTGEGQKLGSTAPQubiquitination[2, 5]
313DGGRLVQKFNHSHRIubiquitination[4]
358ADESQTLKEANLLNAubiquitination[5]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Reduces the ATPase activity of VCP. Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP- mediated formation of transitional endoplasmic reticulum (tER) (By similarity). Inhibits the activity of CTSL (in vitro). 
Sequence Annotation
 DOMAIN 179 244 SEP.
 DOMAIN 291 368 UBX.
 MOTIF 109 115 Nuclear localization signal.
 MOTIF 172 175 Nuclear localization signal.
 MOD_RES 74 74 Phosphoserine (By similarity).
 MOD_RES 114 114 Phosphoserine.
 MOD_RES 140 140 Phosphoserine.
 MOD_RES 167 167 Phosphotyrosine (By similarity).
 MOD_RES 176 176 Phosphoserine (By similarity).
 MOD_RES 272 272 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Chromosome; Complete proteome; Direct protein sequencing; Golgi apparatus; Lipid-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 370 AA 
Protein Sequence
MAAERQEALR EFVAVTGAEE DRARFFLESA GWDLQIALAS FYEDGGDEDI VTISQATPSS 60
VSRGTAPSDN RVTSFRDLIH DQDEDEEEEE GQRSRFYAGG SERSGQQIVG PPRKKSPNEL 120
VDDLFKGAKE HGAVAVERVT KSPGETSKPR PFAGGGYRLG AAPEEESAYV AGEKRQHSSQ 180
DVHVVLKLWK SGFSLDNGEL RSYQDPSNAQ FLESIRRGEV PAELRRLAHG GQVNLDMEDH 240
RDEDFVKPKG AFKAFTGEGQ KLGSTAPQVL STSSPAQQAE NEAKASSSIL IDESEPTTNI 300
QIRLADGGRL VQKFNHSHRI SDIRLFIVDA RPAMAATSFI LMTTFPNKEL ADESQTLKEA 360
NLLNAVIVQR LT 372 
Gene Ontology
 GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
 GO:0005795; C:Golgi stack; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0008289; F:lipid binding; IEA:UniProtKB-KW. 
Interpro
 IPR012989; SEP_domain.
 IPR009060; UBA-like.
 IPR001012; UBX. 
Pfam
 PF08059; SEP
 PF00789; UBX 
SMART
 SM00553; SEP
 SM00166; UBX 
PROSITE
 PS51399; SEP
 PS50033; UBX 
PRINTS