UniProt Accession

 FL2D_HUMAN; Q15007; Q5TCL8; Q5TCL9; Q96T28; Q9BYJ7; Q9H4E2 

Genbank Protein ID

 AF374416; D14661; AK127822; AL135914; AL135914; CH471051; CH471051; CH471051; BC000383; BC004432; BC069192; AJ276706; AL583911 

Genbank Nucleotide ID

 AAK54764.1; BAA03495.1; BAG54579.1; CAI21848.1; CAI21849.1; EAW47622.1; EAW47624.1; EAW47625.1; AAH00383.1; AAH04432.1; AAH69192.1; CAC10188.1; CAC29495.1 

Protein Name

 Pre-mRNA-splicing regulator WTAP 

Protein Synonyms/Alias

 Female-lethal(2)D homolog; hFL(2)D; WT1-associated protein; Wilms tumor 1-associating protein 

Gene Name

 WTAP 

Gene Synonyms/Alias

 KIAA0105 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
9	TNEEPLPKKVRLSET	ubiquitination	[1, 2]
10	NEEPLPKKVRLSETD	ubiquitination	[2]
19	RLSETDFKVMARDEL	ubiquitination	[2, 3, 4, 5]
31	DELILRWKQYEAYVQ	ubiquitination	[2, 6]
43	YVQALEGKYTDLNSN	ubiquitination	[2, 6]
61	GLRESEEKLKQQQQE	ubiquitination	[2]
63	RESEEKLKQQQQESA	ubiquitination	[6]
83	LVMRLATKEQEMQEC	ubiquitination	[2, 5]
98	TTQIQYLKQVQQPSV	ubiquitination	[2, 5, 7]
123	AINLFFLKMKGELEQ	ubiquitination	[2, 3, 4]
125	NLFFLKMKGELEQTK	ubiquitination	[2, 5, 6]
134	ELEQTKDKLEQAQNE	ubiquitination	[6]
146	QNELSAWKFTPDSQT	ubiquitination	[6]

Reference

 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [7] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094] 

Functional Description

 Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA- binding ability and inhibits expression of WT1 target genes. May be involved in mRNA splicing regulation. 

Sequence Annotation

 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 305 305 Phosphoserine.
 MOD_RES 306 306 Phosphoserine.
 MOD_RES 388 388 Phosphoserine.  

Keyword

 Acetylation; Alternative splicing; Cell cycle; Complete proteome; Direct protein sequencing; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 396 AA 

Protein Sequence

Gene Ontology

 GO:0031965; C:nuclear membrane; IDA:UniProtKB.
 GO:0005654; C:nucleoplasm; IEA:Compara.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW. 

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