UniProt Accession

 RTN4_HUMAN; Q9NQC3; O94962; Q7L7Q5; Q7L7Q6; Q7L7Q8; Q8IUA4; Q96B16; Q9BXG5; Q9H212; Q9H3I3; Q9UQ42; Q9Y293; Q9Y2Y7; Q9Y5U6 

Genbank Protein ID

 AF087901; AF148537; AF148538; AJ251383; AJ251384; AJ251385; AB040462; AB040463; AF333336; AY102285; AY102285; AY102285; AY102285; AY102285; AY102276; AY102277; AY102278; AY102279; AY123245; AY123246; AY123247; AY123248; AY123249; AY123250; AB015639; AF077050; AF132047; AF132048; AF320999; AB020693; AF125103; AF177332; AC013414; AC092461; AC093165; CH471053; CH471053; CH471053; CH471053; CH471053; CH471053; CH471053; CH471053; CH471053; CH471053; CH471053; CH471053; CH471053; BC001035; BC007109; BC010737; BC012619; BC014366; BC016165; BC026788; BC068991; BC150182; BC152425; BC152555; AF063601 

Genbank Nucleotide ID

 AAG12205.1; AAG12176.1; AAG12177.1; CAB99248.1; CAB99249.1; CAB99250.1; BAB18927.1; BAB18928.1; AAK20831.1; AAM64240.1; AAM64241.1; AAM64242.1; AAM64243.1; AAM64244.1; AAM64245.1; AAM64246.1; AAM64247.1; AAM64248.1; AAM64249.1; AAM64250.1; AAM64251.1; AAM64252.1; AAM64253.1; AAM64254.1; BAA83712.1; AAD27783.1; AAD31021.1; AAD31022.1; AAG40878.1; BAA74909.2; AAD39920.1; AAG17976.1; EAX00115.1; EAX00116.1; EAX00117.1; EAX00118.1; EAX00119.1; EAX00121.1; EAX00122.1; EAX00123.1; EAX00124.1; EAX00125.1; EAX00127.1; EAX00130.1; EAX00132.1; AAH01035.1; AAH07109.1; AAH10737.1; AAH12619.1; AAH14366.1; AAH16165.1; AAH26788.1; AAH68991.1; AAI50183.1; AAI52426.1; AAI52556.1; AAG43160.1 

Protein Name

 Reticulon-4 

Protein Synonyms/Alias

 Foocen; Neurite outgrowth inhibitor; Nogo protein; Neuroendocrine-specific protein; NSP; Neuroendocrine-specific protein C homolog; RTN-x; Reticulon-5 

Gene Name

 RTN4 

Gene Synonyms/Alias

 KIAA0886; NOGO; My043; SP1507 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
58	ELEVLERKPAAGLSA	ubiquitination	[1]
352	ELPTALTKLVKEDEV	ubiquitination	[2, 3]
364	DEVVSSEKAKDSFNE	ubiquitination	[2, 3]
1066	GVIQAIQKSDEGHPF	ubiquitination	[1]
1090	ISEELVQKYSNSALG	ubiquitination	[1]
1104	GHVNCTIKELRRLFL	acetylation	[4]
1104	GHVNCTIKELRRLFL	ubiquitination	[2, 3, 5, 6]
1171	HYLGLANKNVKDAMA	ubiquitination	[1, 6]
1174	GLANKNVKDAMAKIQ	ubiquitination	[1]
1179	NVKDAMAKIQAKIPG	ubiquitination	[1]
1183	AMAKIQAKIPGLKRK	ubiquitination	[6]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex (By similarity). Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing. 

Sequence Annotation

 DOMAIN 1005 1192 Reticulon.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 7 7 Phosphoserine.
 MOD_RES 15 15 Phosphoserine.
 MOD_RES 107 107 Phosphoserine.
 MOD_RES 152 152 Phosphoserine (By similarity).
 MOD_RES 181 181 Phosphoserine.
 MOD_RES 182 182 Phosphoserine.
 MOD_RES 188 188 Phosphothreonine (By similarity).
 MOD_RES 361 361 Phosphoserine (By similarity).
 MOD_RES 446 446 Phosphoserine (By similarity).
 MOD_RES 860 860 Phosphoserine (By similarity).
 MOD_RES 863 863 Phosphoserine (By similarity).
 MOD_RES 889 889 Phosphotyrosine (By similarity).
 MOD_RES 1104 1104 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Membrane; Neurogenesis; Phosphoprotein; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1192 AA 

Protein Sequence

Gene Ontology

 GO:0042995; C:cell projection; IEA:Compara.
 GO:0030176; C:integral to endoplasmic reticulum membrane; IDA:UniProtKB.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0005635; C:nuclear envelope; IDA:UniProtKB.
 GO:0005886; C:plasma membrane; TAS:Reactome.
 GO:0001525; P:angiogenesis; IEA:Compara.
 GO:0006915; P:apoptotic process; NAS:UniProtKB.
 GO:0007413; P:axonal fasciculation; ISS:UniProtKB.
 GO:0060317; P:cardiac epithelial to mesenchymal transition; IEA:Compara.
 GO:0021801; P:cerebral cortex radial glia guided migration; ISS:UniProtKB.
 GO:0071786; P:endoplasmic reticulum tubular network organization; IMP:UniProtKB.
 GO:0030517; P:negative regulation of axon extension; IDA:UniProtKB.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0042981; P:regulation of apoptotic process; NAS:UniProtKB.
 GO:2000172; P:regulation of branching morphogenesis of a nerve; ISS:UniProtKB.
 GO:0030334; P:regulation of cell migration; IDA:MGI. 

Interpro

 IPR003388; Reticulon. 

Pfam

 PF02453; Reticulon 

SMART

  

PROSITE

 PS50845; RETICULON 

PRINTS