UniProt Accession

 T2FB_MOUSE; Q8R0A0 

Genbank Protein ID

 BC027173 

Genbank Nucleotide ID

 AAH27173.1 

Protein Name

 General transcription factor IIF subunit 2 

Protein Synonyms/Alias

 ATP-dependent helicase GTF2F2; Transcription initiation factor IIF subunit beta; TFIIF-beta 

Gene Name

 Gtf2f2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
33	YLSQQWAKASGRGEV	ubiquitination	[1]
71	NIHDIGGKPASVSAP	acetylation	[2]
137	LQIEESSKPVRLSQQ	acetylation	[3]
154	KVVTTNYKPVANHQY	acetylation	[2, 3, 4]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 

Functional Description

 TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA- helicase activity (By similarity). 

Sequence Annotation

 NP_BIND 36 43 ATP (Potential).
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 22 22 N6-acetyllysine (By similarity).
 MOD_RES 33 33 N6-acetyllysine (By similarity).
 MOD_RES 137 137 N6-acetyllysine (By similarity).
 MOD_RES 142 142 Phosphoserine (By similarity).
 MOD_RES 248 248 Phosphoserine (By similarity).  

Keyword

 Acetylation; ATP-binding; Complete proteome; DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 249 AA 

Protein Sequence

Gene Ontology

 GO:0015630; C:microtubule cytoskeleton; IEA:Compara.
 GO:0005674; C:transcription factor TFIIF complex; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006367; P:transcription initiation from RNA polymerase II promoter; IEA:InterPro. 

Interpro

 IPR016640; TFIIF-beta_subgr.
 IPR003196; TFIIF_beta.
 IPR011039; TFIIF_interaction.
 IPR011991; WHTH_DNA-bd_dom. 

Pfam

 PF02270; TFIIF_beta 

SMART

  

PROSITE

  

PRINTS