UniProt Accession

 MNME_ECOLI; P25522; Q2M819 

Genbank Protein ID

 S57109; L10328; U00096; AP009048 

Genbank Nucleotide ID

 AAB19981.1; AAA62057.1; AAC76729.1; BAE77587.1 

Protein Name

 tRNA modification GTPase MnmE 

Protein Synonyms/Alias

  

Gene Name

 mnmE 

Gene Synonyms/Alias

 thdF; trmE; b3706; JW3684 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
327	FIARLPAKLPITVVR	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34. 

Sequence Annotation

 DOMAIN 218 336 G.
 NP_BIND 226 231 GTP.
 NP_BIND 245 251 GTP.
 NP_BIND 270 273 GTP.
 NP_BIND 335 338 GTP.
 NP_BIND 358 360 GTP.
 METAL 226 226 Potassium.
 METAL 230 230 Magnesium.
 METAL 245 245 Potassium; via carbonyl oxygen.
 METAL 247 247 Potassium; via carbonyl oxygen.
 METAL 250 250 Potassium.
 METAL 251 251 Magnesium.
 BINDING 23 23 Formyltetrahydrofolate (By similarity).
 BINDING 80 80 Formyltetrahydrofolate (By similarity).
 BINDING 120 120 Formyltetrahydrofolate (By similarity).
 BINDING 454 454 Formyltetrahydrofolate (By similarity).  

Keyword

 3D-structure; Complete proteome; Cytoplasm; GTP-binding; Hydrolase; Magnesium; Metal-binding; Nucleotide-binding; Potassium; Reference proteome; tRNA processing. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 454 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:EcoCyc.
 GO:0005525; F:GTP binding; IDA:EcoCyc.
 GO:0003924; F:GTPase activity; IDA:EcoCyc.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0009268; P:response to pH; IMP:EcoCyc.
 GO:0030488; P:tRNA methylation; IMP:EcoCyc.
 GO:0006805; P:xenobiotic metabolic process; IMP:EcoCyc. 

Interpro

 IPR018948; GTP-bd_TrmE_N.
 IPR006073; GTP_binding_domain.
 IPR004520; GTPase_MnmE.
 IPR025867; GTPase_MnmE_C_dom.
 IPR027368; MnmE_dom2.
 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR027266; TrmE/GcvT_dom1. 

Pfam

 PF12631; GTPase_Cys_C
 PF01926; MMR_HSR1
 PF10396; TrmE_N 

SMART

  

PROSITE

  

PRINTS