CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024374
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 MAX gene-associated protein 
Protein Synonyms/Alias
  
Gene Name
 Mga 
Gene Synonyms/Alias
 Kiaa4252 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
705TRISQLEKELIEDLKubiquitination[1]
795KIKGWRGKFQNASASacetylation[2]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
 Functions as a dual-specificity transcription factor, regulating the expression of both MAX-network and T-box family target genes. Functions as a repressor or an activator. Binds to 5'-AATTTCACACCTAGGTGTGAAATT-3' core sequence and seems to regulate MYC-MAX target genes. Suppresses transcriptional activation by MYC and inhibits MYC-dependent cell transformation. Function activated by heterodimerization with MAX. This heterodimerization serves the dual function of both generating an E-box-binding heterodimer and simultaneously blocking interaction of a corepressor. 
Sequence Annotation
 DOMAIN 2362 2413 bHLH.
 DNA_BIND 84 260 T-box.
 MOD_RES 531 531 Phosphoserine (By similarity).
 MOD_RES 921 921 Phosphoserine (By similarity).
 MOD_RES 1423 1423 Phosphoserine (By similarity).
 MOD_RES 1450 1450 Phosphoserine (By similarity).
 MOD_RES 2480 2480 Phosphoserine (By similarity).
 MOD_RES 2849 2849 Phosphoserine (By similarity).
 MOD_RES 2860 2860 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; Coiled coil; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 3003 AA 
Protein Sequence
MEEKQQIILA NQDGGTVTGG APTFFVILKQ PGNGKTDQGI LVTNRDARAL LSRESSPGKS 60
KEKICLPADC TVGKITVTLD NNSMWNEFHN RSTEMILTKQ GRRMFPYCRY WITGLDSNLK 120
YILVMDISPV DSHRYKWNGR WWEPSGKAEP HILGRVFIHP ESPSTGHYWM HQPVSFYKLK 180
LTNNTLDQEG HIILHSMHRY LPRLHLVPAE KATEVIQLNG PGVHTFTFPQ TEFFAVTAYQ 240
NIQITQLKID YNPFAKGFRD DGLSSKPQRE GKQRNSSDQE GNSVSSSPAH RVRLTEGEGS 300
EIHSGDFDPV LRGHEASSLS LEKAPHNVKQ DFLGFMNTDS THEVPQLKHE ISESRIVNSF 360
EDDSQISSPS NPNGNFNVVI KEEPLDDYDY ELGECPEGIT VKQEETDEET DVYSNSDDDP 420
ILEKQLKRHN KVDNLEADHP SYKWLPNSPG VAKAKMFKLD AGKMPVVYLE PCAVTKSTVK 480
ISELPDNMLS TSRKDKSMLA ELEYLPAYIE NSDGTDFCLS KDSENSLRKH SPDLRIVQKY 540
TLLKEPNWKY PDILDNSSTE RIHDSSKGST AESFSGKEDL GKKRTTMLKM AIPSKTVTAS 600
HSASPNTPGK RGRPRKLRLS KAGRPPKNTG KSLTAAKNIP VGPGSTFPDV KPDLEDVDGV 660
LFVSFESKEA LDIHAVDGTT EEPSSLQTTT TNDSGCRTRI SQLEKELIED LKSLRHKQVI 720
HPALQEVGLK LNSVDPTVSI DLKYLGVQLP LAPATSFPLW NVTGTNPASP DAGFPFVSRT 780
GKTNDFTKIK GWRGKFQNAS ASRNEGGNSE ASLKNRSAFC SDKLDEYLEN EGKLMETNIG 840
FSSNAPTSPV VYQLPTKSTS YVRTLDSVLK KQSTISPSTS HSVKPQSVTT ASRKTKAQNK 900
QTTLSGRTKS SYKSILPYPV SPKQKNSHVS QGDKITKNSL SSTSDNQVTN LVVPSVDENA 960
FPKQISLRQA QQQHLQQQGT RPPGLSKSQV KLMDLEDCAL WEGKPRTYIT EERADVSLTT 1020
LLTAQASLKT KPIHTIIRKR APPCNNDFCR LGCVCSSLAL EKRQPAHCRR PDCMFGCTCL 1080
KRKVVLVKGG SKTKHFHKKA ANRDPLFYDT LGEEGREGGG VREDEEQLKE KKKRKKLEYT 1140
VCEAEPEQPV RHYPLWVKVE GEVDPEPVYI PTPSVIEPIK PLVLPQPDLS STTKGKLTPG 1200
IKPARTYTPK PNPVIREEDK DPVYLYFESM MTCARVRVYE RKKEEQRQLS PPLSPSSSFQ 1260
QQSSCYSSPE NRVTKELDSE QTLKQLICDL EDDSDKSQEK SWKSSCNEGE SSSTSYVHQR 1320
SPGGPTKLIE IISDCNWEED RNKILSILSQ HINSNMPQSL KVGSFIIELA SQRKCRGEKT 1380
PPVYSSRVKI SMPSSQDQDD MAEKSGSETP DGPLSPGKMD DISPVQTDAL DSVRERLHGG 1440
KGLPFYAGLS PSGKLVAYKR KPSSTTSGLI QVASNAKVAA SRKPRTLLPS TSNSKMASSG 1500
PATNRSGKNL KAFVPAKRPI AARPSPGGVF TQFVMSKVGA LQQKIPGVRT PQPLTGPQKF 1560
SIRPSPVMVV TPVVSSEQVQ VCSTVAAAVT TSPQVFLENV TAVPSLTANS DMGAKEATYS 1620
SSASTAGVVE ISETNNTTLV TSTQSTATVN LTKTTGITTS PVASVSFAKP LVASPTITLP 1680
VASTASTSIV MVTTAASSSV VTTPTSSLSS VPIILSGING SPPVSQRPEN APQIPVTTPQ 1740
ISSNNVKRTG PRLLHPNGQI VQLLPLHQIR GSNAQPSLQP VVFRNPGSMV GIRLPAPCKS 1800
SETPSSSASS SAFSVMSPVI QAVGSSPTVN VISQAPSLLS SGSSFVSQAG TLTLRISPPE 1860
TQNLASKTGS ESKITPSTGG QPVGTASLIP LQSGSFALLQ LPGQKPIPSS VLQHVASLQI 1920
KKESQSTDQK DETNSIKREE ETKKALPSKD KALDSEANIM KQNSGIIASE NTSNNSLDDG 1980
GDLLDEETLR EDARPYEYSY STGSHTDEDK DGDEDSGNKN QNSPKEKQTV PEVRAGSKNI 2040
DIMALQSIRS IRPQKCVKVK VEPQEGSDNP ENPDDFLVLS KDSKFELSGN QVKEQQSNSQ 2100
AEAKKDCEDS LGKDSLRERW RKHLKGPLTQ KYIGISQNFN KEANVQFFTE MKPCQENSEQ 2160
DISELLGKSG TIESGGVLKT EDGSWSGISS SAAFSIIPRR ATKGRRGSRH FQGHLLLPRE 2220
QMKPKQQTKD GRSSAADFTV LDLEDEDEED EKTDDSLDEI VDVVSGYQSE EVDVEKNNYV 2280
DYLEDDEQVD VETIEELSEE INFPYKKTTA THTQSFKQQC HSHISADEKA SEKSRKVSLI 2340
SSKLKDDCWG DKPHKETEAF AYYRRTHTAN ERRRRGEMRD LFEKLKITLG LLHSSKVSKS 2400
LILNRAFSEI QGLTDQADKL IGQKNLLSRK RSILIRKVSS LSGKTEEVVL KKLEYIYAKQ 2460
QALEAQKRKK KLGSDEFCVS PRIGTQLEGS SASSVDLGQM LMNNRRGKPL ILSRKRDQAT 2520
ENASPSDTPH SSANLVMTPQ GQLLTLKGPL FSGPVVAVSP ALLEGGLKPQ VASSTMSQSE 2580
NDDLFMMPRI VNVTSLAAEE DLGGMSGNKY RHEVPDGKPL DHLRDIAGSE ASSLKDTERI 2640
SSRGNHRDSR KALGPTQVLL ANKDSGFPHV ADVSTMQAAQ EFIPKNMSGD VRGHRYKWKE 2700
CELRGERLKS KESQFHKLKM KDLKDSSIEM ELRKVASAIE EAALHPSELL TNMEDEDDTD 2760
ETLTSLLNEI AFLNQQLNDD SGLAELSGSM DTEFSGDAQR AFISKLAPGN RSAFQVGHLG 2820
AGVKELPDVQ EESESISPLL LHLEDDDFSE NEKQLGDTAS EPDVLKIVID PEIKDSLVSH 2880
RKSSDGGQST SGLPAEPESV SSPPILHMKT GPENSNTDTL WRPMPKLAPL GLKVANPPSD 2940
ADGQSLKVMP ALAPIAAKVG SIGHKMNLAG IDQEGRGSKV MPTLAPVVPK LGNSGAPSSS 3000
SGK 3003 
Gene Ontology
 GO:0071339; C:MLL1 complex; ISS:UniProtKB.
 GO:0005667; C:transcription factor complex; IDA:MGI.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:MGI.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR011598; bHLH_dom.
 IPR008967; p53-like_TF_DNA-bd.
 IPR001699; TF_T-box.
 IPR018186; TF_T-box_CS. 
Pfam
 PF00010; HLH
 PF00907; T-box 
SMART
 SM00353; HLH
 SM00425; TBOX 
PROSITE
 PS50888; BHLH
 PS01283; TBOX_1
 PS01264; TBOX_2
 PS50252; TBOX_3 
PRINTS
 PR00937; TBOX.