CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006436
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Antiviral helicase SKI2 
Protein Synonyms/Alias
 Superkiller protein 2 
Gene Name
 SKI2 
Gene Synonyms/Alias
 YLR398C; L8084.17 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
168GIPMKSVKTDSEQNGubiquitination[1]
900PSILHILKEGRLVAFacetylation[2]
953NHLIYFPKADGYRRRacetylation[2]
1184RIEEIYKKMLCVFNTubiquitination[3]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 RNA helicase component of the SKI complex involved in 3'-mRNA degradation pathway. Represses dsRNA virus propagation by specifically blocking translation of viral mRNAs, perhaps recognizing the absence of CAP or poly(A). Essential for cell growth only in the presence of M1 replicon. 
Sequence Annotation
 DOMAIN 338 496 Helicase ATP-binding.
 DOMAIN 638 815 Helicase C-terminal.
 NP_BIND 351 358 ATP (Potential).
 REGION 556 577 RNA-binding RGG-box (By similarity).
 MOTIF 444 447 DEVH box.
 MOD_RES 209 209 Phosphoserine.  
Keyword
 3D-structure; Antiviral defense; ATP-binding; Complete proteome; Cytoplasm; DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Phosphoprotein; Reference proteome; RNA-binding; Translation regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1287 AA 
Protein Sequence
MSEGFSSSSI QELYQSLKEI TNNADVELFE DRITKLDFES TDEPKHANDI IKDRFLRPSN 60
ALPWSLLDMV QDVPHTSSPE DCSGKLDYKE LLKVPDPINR TSYQFKRTGL EGKISGYKEE 120
VDLKEVANAN ASNSLSITRS INHNQNSVRG STAQLPFTPG GIPMKSVKTD SEQNGSSTMA 180
NATKLLHKDG QGLFDIPEGM NRGIKPMDSP AENEDQNGQF KELKQLNEID NELDIRIEAN 240
EAKLKEEEKS AKSISEEIME EATEETTADN ADDAEIDELL PIGIDFGRTK PVSKSVPVKK 300
EWAHVVDLNH KIENFDELIP NPARSWPFEL DTFQKEAVYH LEQGDSVFVA AHTSAGKTVV 360
AEYAIAMAHR NMTKTIYTSP IKALSNQKFR DFKETFDDVN IGLITGDVQI NPDANCLIMT 420
TEILRSMLYR GADLIRDVEF VIFDEVHYVN DQDRGVVWEE VIIMLPQHVK FILLSATVPN 480
TYEFANWIGR TKQKNIYVIS TPKRPVPLEI NIWAKKELIP VINQNSEFLE ANFRKHKEIL 540
NGESAKGAPS KTDNGRGGST ARGGRGGSNT RDGRGGRGNS TRGGANRGGS RGAGAIGSNK 600
RKFFTQDGPS KKTWPEIVNY LRKRELLPMV VFVFSKKRCE EYADWLEGIN FCNNKEKSQI 660
HMFIEKSITR LKKEDRDLPQ ILKTRSLLER GIAVHHGGLL PIVKELIEIL FSKGFIKVLF 720
ATETFAMGLN LPTRTVIFSS IRKHDGNGLR ELTPGEFTQM AGRAGRRGLD STGTVIVMAY 780
NSPLSIATFK EVTMGVPTRL QSQFRLTYNM ILNLLRIEAL RVEEMIKYSF SENAKETLQP 840
EHEKQIKVLQ EELQTIEYKS CEICDNDIEK FLELMLAYKE ATVNLMQEMV KSPSILHILK 900
EGRLVAFRDP NDCLKLGFVF KVSLKDAVCV IMTFTKPYKL PNGEPNHLIY FPKADGYRRR 960
NFPKFQKTDF YMEEVPVTAI EVITKRKFAA PLGKVIKKDV AALNEFNAET NNILDGKTLK 1020
EAINIEKQGL KIHQILLDRT NIRDEIFKLK SIKCPNLSQH IVPKFKAHVI KKKIEELYHL 1080
MSDQNLSLLP DYEKRLAVLK DTEFIDQNHN VLLKGRVACE INSGYELVLT ELILDNFLGS 1140
FEPEEIVALL SVFVYEGKTR EEEPPIVTPR LAKGKQRIEE IYKKMLCVFN THQIPLTQDE 1200
AEFLDRKRFA MMNVVYEWAR GLSFKEIMEM SPEAEGTVVR VITWLDEICR EVKTASIIIG 1260
NSTLHMKMSR AQELIKRDIV FAASLYL 1287 
Gene Ontology
 GO:0055087; C:Ski complex; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0003724; F:RNA helicase activity; ISS:SGD.
 GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
 GO:0070478; P:nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay; IMP:SGD.
 GO:0070481; P:nuclear-transcribed mRNA catabolic process, non-stop decay; IMP:SGD.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW. 
Interpro
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR012961; DSH_C.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR016438; RNA_helicase_ATP-dep_SK12/DOB1.
 IPR025696; rRNA_proc-arch_dom. 
Pfam
 PF00270; DEAD
 PF08148; DSHCT
 PF00271; Helicase_C
 PF13234; rRNA_proc-arch 
SMART
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 
PRINTS