| Tag | Content |
|---|
| CPLM ID | CPLM-007613 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Elongator complex protein 2 |
Protein Synonyms/Alias | Gamma-toxin target 2 |
Gene Name | ELP2 |
Gene Synonyms/Alias | TOT2; YGR200C; G7725 |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 147 | LAHEFTIKKGFFYPL | acetylation | [1] |
|
Reference | [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae. Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C. Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [ PMID: 22865919] |
Functional Description | Acts as component of the RNA polymerase II elongator complex, which is a major histone acetyltransferase component of the RNA polymerase II (RNAPII) holoenzyme and is involved in transcriptional elongation. Association with elongating RNAPII requires a hyperphosphorylated state of the RNAPII C-terminal domain (CTD). Elongator binds to both naked and nucleosomal DNA, can acetylate both core and nucleosomal histones, and is involved in chromatin remodeling. It acetylates histones H3, preferentially at 'Lys-14', and H4, preferentially at 'Lys-8'. It functions as a gamma-toxin target (TOT); disruption of the complex confers resistance to Kluyveromyces lactis toxin zymocin (pGKL1 killer toxin). May also be involved in sensitiviy to Pichia inositovora toxin. May be involved in tRNA modification. ELP2 is dispensable for the complex integrity and, in vitro, is not required for complex HAT activity. It is not required for the association of the complex with nascent RNA transcript. Independently, ELP2 may be involved in polarized exocytosis. Is required for an early step in synthesis of 5-methoxycarbonylmethyl (mcm5) and 5- carbamoylmethyl (ncm5) groups present on uridines at the wobble position in tRNA. |
Sequence Annotation | REPEAT 57 87 WD 1.
REPEAT 101 130 WD 2.
REPEAT 141 181 WD 3.
REPEAT 200 234 WD 4.
REPEAT 279 309 WD 5.
REPEAT 383 413 WD 6.
REPEAT 438 466 WD 7.
REPEAT 604 634 WD 8.
REPEAT 651 683 WD 9. |
Keyword | Complete proteome; Cytoplasm; Nucleus; Protein transport; Reference proteome; Repeat; Transcription; Transcription regulation; Transport; WD repeat. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 788 AA |
Protein Sequence | MVECITPEAI FIGANKQTQV SDIHKVKKIV AFGAGKTIAL WDPIEPNNKG VYATLKGHEA 60
EVTCVRFVPD SDFMVSASED HHVKIWKFTD YSHLQCIQTI QHYSKTIVAL SALPSLISVG 120
CADGTISIWR QNIQNDEFGL AHEFTIKKGF FYPLCLSLSK VEEKKYLLAI GGTNVNVFIA 180
SFILSDSGIE KCRVVAELEG HEDWVKSLAF RHQETPGDYL LCSGSQDRYI RLWRIRINDL 240
IDDSEEDSKK LTLLSNKQYK FQIDDELRVG INFEALIMGH DDWISSLQWH ESRLQLLAAT 300
ADTSLMVWEP DETSGIWVCS LRLGEMSSKG ASTATGSSGG FWSCLWFTHE RMDFFLTNGK 360
TGSWRMWATK DNIICDQRLG ISGATKDVTD IAWSPSGEYL LATSLDQTTR LFAPWIYDAS 420
GRKREIATWH EFSRPQIHGY DMICVETVTD TRFVSGGDEK ILRSFDLPKG VAGMLQKFVG 480
IQFEEKSEMP DSATVPVLGL SNKAGEDDAN EDDEEEEGGN KETPDITDPL SLLECPPMED 540
QLQRHLLWPE VEKLYGHGFE ITCLDISPDQ KLIASACRSN NVQNAVIRIF STENWLEIKP 600
ALPFHSLTIT RLKFSKDGKF LLSVCRDRKW ALWERNMEDN TFELRFKNEK PHTRIIWDAD 660
WAPLEFGNVF VTASRDKTVK VWRHQKEPAD DYVLEASIKH TKAVTAISIH DSMIREKILI 720
SVGLENGEIY LYSYTLGKFE LITQLNEDIT PADKITRLRW SHLKRNGKLF LGVGSSDLST 780
RIYSLAYE 788 |
Gene Ontology | GO:0005737; C:cytoplasm; IDA:SGD. GO:0033588; C:Elongator holoenzyme complex; IDA:SGD. GO:0005634; C:nucleus; IDA:SGD. GO:0015031; P:protein transport; IEA:UniProtKB-KW. GO:0032447; P:protein urmylation; IMP:SGD. GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IMP:SGD. GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. GO:0002098; P:tRNA wobble uridine modification; IMP:SGD. |
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