CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029211
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E1A-binding protein p400 
Protein Synonyms/Alias
 EP400 protein 
Gene Name
 EP400 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
345SVGNTGMKKVPKKLEacetylation[1]
346VGNTGMKKVPKKLEEubiquitination[2]
863LRVELEEKRKKALNLubiquitination[2]
872KKALNLQKVSRRGKEubiquitination[2]
1034VAEAILPKGSARVTTubiquitination[2]
1060GALRDYQKIGLDWLAubiquitination[3]
1255ESQDYYHKVVIRLHRubiquitination[2, 3, 4, 5]
1278RTKRDVEKQLTKKYEubiquitination[2]
1283VEKQLTKKYEHVLKCubiquitination[2]
1297CRLSNRQKALYEDVIubiquitination[2]
1362PSASLILKALERDFWubiquitination[2, 4, 6, 7]
1385DLIGLENKITRHEAEubiquitination[2, 4]
1402SKKKIPRKLMEEISTubiquitination[7]
1436QPVQYGQKPEGRTVAacetylation[1, 8]
1436QPVQYGQKPEGRTVAubiquitination[2]
1517TLQFQGSKFTLSHSQacetylation[1, 5]
1517TLQFQGSKFTLSHSQubiquitination[2, 3]
1612GEPGTASKPASPIGGubiquitination[2, 4]
1678SLDGRRGKEAGPAHSubiquitination[2]
Reference
 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1731 AA 
Protein Sequence
MHHGTGPQNV QHQLQRSRAC PGSEGEEQPA HPNPPPSPAA PFAPSASPSA PQSPSYQIQQ 60
LMNRSPATGQ NVNITLQSVG PVVGGNQQIT LAPLPLPSPT SPGFQFSAQP RRFEHGSPSY 120
IQVTSPLSQQ VQTQSPTQPS PGPGQALQNV RAGAPGPGLG LCSSSPTGGF VDASVLVRQI 180
SLSPSSGGHF VFQDGSGLTQ IAQGAQVQLQ HPGTPITVRE RRPSQPHTQS GGTIHHLGPQ 240
SPAAAGGAGL QPLASPSHIT TANLPPQISS IIQGQLVQQQ QVLQGPPLPR PLGFERTPGV 300
LLPGAGGAAG FGMTSPPPPT SPSRTAVPPG LSSLPLTSVG NTGMKKVPKK LEEIPPASPE 360
MAQMRKQCLD YHYQEMQALK EVFKEYLIEL FFLQHFQGNM MDFLAFKKKH YAPLQAYLRQ 420
NDLDIEEEEE EEEEEEEKSE VINDEQQALA GSLVAGAGST VETDLFKRQQ AMPSTGMAEQ 480
SKRPRLEVGH QGVVFQHPGA DAGVPLQQLM PTAQGGMPPT PQAAQLAGQR QSQQQYDPST 540
GPPVQNAASL HTPLPQLPGR LPPAGVPTAA LSSALQFAQQ PQVVEAQTQL QIPVKTQQPN 600
VPIPAPPSSQ LPIPPSQPAQ LALHVPTPGK VQVQASQLSS LPQMVASTRL PVDPAPPCPR 660
PLPTSSTSSL APVSGSGPGP SPARSSPVNR PSSATNKALS PVTSRTPGVV ASAPTKPQSP 720
AQNATSSQDS SQDTLTEQIT LENQVHQRIA ELRKAGLWSQ RRLPKLQEAP RPKSHWDYLL 780
EEMQWMATDF AQERRWKVAA AKKLVRTVVR HHEEKQLREE RGKKEEQSRL RRIAASTARE 840
IECFWSNIEQ VVEIKLRVEL EEKRKKALNL QKVSRRGKEL RPKGFDALQE SSLDSGMSGR 900
KRKASISLTD DEVDDEEETI EEEEANEGVV DHQTELSNLA KEAELPLLDL MKLYEGAFLP 960
SSQWPRPKPD GEDTSGEEDA DDCPGDRESR KDLVLIDSLF IMDQFKAAER MNIGKPNAKD 1020
IADVTAVAEA ILPKGSARVT TSVKFNAPSL LYGALRDYQK IGLDWLAKLY RKNLNGILAD 1080
EAGLGKTVQI IAFFAHLACN EGNWGPHLVV VRSCNILKWE LELKRWCPGL KILSYIGSHR 1140
ELKAKRQEWA EPNSFHVCIT SYTQFFRGLT AFTRVRWKCL VIDEMQRVKG MTERHWEAVF 1200
TLQSQQRLLL IDSPLHNTFL ELWTMVHFLV PGISRPYLSS PLRAPSEESQ DYYHKVVIRL 1260
HRVTQPFILR RTKRDVEKQL TKKYEHVLKC RLSNRQKALY EDVILQPGTQ EALKSGHFVN 1320
VLSILVRLQR ICNHPGLVEP RHPGSSYVAG PLEYPSASLI LKALERDFWK EADLSMFDLI 1380
GLENKITRHE AELLSKKKIP RKLMEEISTS AAPAARPAAA KLKASRLFQP VQYGQKPEGR 1440
TVAFPSTHPP RTAAPTTASA APQGPLRGRP PIATFSANPE AKGGEVVKIA QLASITGPQS 1500
RVAQPETPVT LQFQGSKFTL SHSQLRQLTA GQPLQLQGSV LQIVSAPGQP YLRAPGPVVM 1560
QTVSQAGAVH GALGSKPPAG GPSPAPLTPQ VGVPGRVAVN ALAVGEPGTA SKPASPIGGP 1620
TQEEKTRLLK ERLDQIYLVN ERRCSQAPVY GRDLLRICAL PSHGRVQWRG SLDGRRGKEA 1680
GPAHSYTSSS ESPSELMLTL CRCGESLQDV IDRVAFVIPP VVAAPPSLRV P 1731 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0003677; F:DNA binding; IEA:InterPro. 
Interpro
 IPR013999; HAS_subgr.
 IPR014012; Helicase/SANT-assoc_DNA-bd.
 IPR014001; Helicase_ATP-bd.
 IPR006562; HSA.
 IPR027417; P-loop_NTPase.
 IPR000330; SNF2_N. 
Pfam
 PF07529; HSA
 PF00176; SNF2_N 
SMART
 SM00487; DEXDc
 SM00573; HSA 
PROSITE
 PS51192; HELICASE_ATP_BIND_1
 PS51204; HSA 
PRINTS