UniProt Accession

 RPAC1_HUMAN; O15160; O75395; Q5JTE3 

Genbank Protein ID

 AF008442; AF047441; AL355802; BC008863 

Genbank Nucleotide ID

 AAC14354.1; AAC39892.1; CAI42629.1; AAH08863.1 

Protein Name

 DNA-directed RNA polymerases I and III subunit RPAC1 

Protein Synonyms/Alias

 DNA-directed RNA polymerase I subunit C; RNA polymerases I and III subunit AC1; AC40; DNA-directed RNA polymerases I and III 40 kDa polypeptide; RPA40; RPA39; RPC40 

Gene Name

 POLR1C 

Gene Synonyms/Alias

 POLR1E 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
47	WDQDRFEKNFRVDVV	ubiquitination	[1]
91	VPTMAVEKVLVYNNT	ubiquitination	[2]
154	TRNPHAAKDSSDPNE	ubiquitination	[1, 2, 3]
167	NELYVNHKVYTRHMT	ubiquitination	[1, 2]
220	HCVKGIGKDHAKFSP	ubiquitination	[2]
224	GIGKDHAKFSPVATA	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8]
270	EVQEVQGKKVARVAN	ubiquitination	[2, 5]
271	VQEVQGKKVARVANP	ubiquitination	[2]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity). 

Sequence Annotation

 MOD_RES 2 2 N-acetylalanine.  

Keyword

 Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; Disease mutation; DNA-directed RNA polymerase; Nucleus; Reference proteome; Transcription. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 346 AA 

Protein Sequence

Gene Ontology

 GO:0005736; C:DNA-directed RNA polymerase I complex; TAS:ProtInc.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003899; F:DNA-directed RNA polymerase activity; TAS:ProtInc.
 GO:0006363; P:termination of RNA polymerase I transcription; TAS:Reactome.
 GO:0006386; P:termination of RNA polymerase III transcription; TAS:Reactome.
 GO:0006362; P:transcription elongation from RNA polymerase I promoter; TAS:Reactome.
 GO:0006385; P:transcription elongation from RNA polymerase III promoter; TAS:Reactome.
 GO:0006361; P:transcription initiation from RNA polymerase I promoter; TAS:Reactome. 

Interpro

 IPR001514; DNA-dir_RNA_pol_30-40kDasu_CS.
 IPR011261; DNA-dir_RNA_pol_dimersation.
 IPR011262; DNA-dir_RNA_pol_insert.
 IPR009025; DNA-dir_RNA_pol_RBP11-like.
 IPR011263; DNA-dir_RNA_pol_RpoA/D/Rpb3. 

Pfam

 PF01000; RNA_pol_A_bac
 PF01193; RNA_pol_L 

SMART

 SM00662; RPOLD 

PROSITE

 PS00446; RNA_POL_D_30KD 

PRINTS