CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007544
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Signal transducer and activator of transcription 1-alpha/beta 
Protein Synonyms/Alias
 Transcription factor ISGF-3 components p91/p84 
Gene Name
 STAT1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
138QSTVMLDKQKELDSKubiquitination[1]
592ERERALLKDQQPGTFubiquitination[1]
636HAVEPYTKKELSAVTubiquitination[1]
652PDIIRNYKVMAAENIubiquitination[1]
665NIPENPLKYLYPNIDubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN- gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. 
Sequence Annotation
 DOMAIN 573 670 SH2.
 MOD_RES 701 701 Phosphotyrosine; by JAK1, JAK2 or TYK2.
 MOD_RES 727 727 Phosphoserine; by MAPK14.
 CROSSLNK 703 703 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Activator; Alternative splicing; Antiviral defense; Complete proteome; Cytoplasm; Direct protein sequencing; Disease mutation; DNA-binding; Host-virus interaction; Isopeptide bond; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; SH2 domain; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 750 AA 
Protein Sequence
MSQWYELQQL DSKFLEQVHQ LYDDSFPMEI RQYLAQWLEK QDWEHAANDV SFATIRFHDL 60
LSQLDDQYSR FSLENNFLLQ HNIRKSKRNL QDNFQEDPIQ MSMIIYSCLK EERKILENAQ 120
RFNQAQSGNI QSTVMLDKQK ELDSKVRNVK DKVMCIEHEI KSLEDLQDEY DFKCKTLQNR 180
EHETNGVAKS DQKQEQLLLK KMYLMLDNKR KEVVHKIIEL LNVTELTQNA LINDELVEWK 240
RRQQSACIGG PPNACLDQLQ NWFTIVAESL QQVRQQLKKL EELEQKYTYE HDPITKNKQV 300
LWDRTFSLFQ QLIQSSFVVE RQPCMPTHPQ RPLVLKTGVQ FTVKLRLLVK LQELNYNLKV 360
KVLFDKDVNE RNTVKGFRKF NILGTHTKVM NMEESTNGSL AAEFRHLQLK EQKNAGTRTN 420
EGPLIVTEEL HSLSFETQLC QPGLVIDLET TSLPVVVISN VSQLPSGWAS ILWYNMLVAE 480
PRNLSFFLTP PCARWAQLSE VLSWQFSSVT KRGLNVDQLN MLGEKLLGPN ASPDGLIPWT 540
RFCKENINDK NFPFWLWIES ILELIKKHLL PLWNDGCIMG FISKERERAL LKDQQPGTFL 600
LRFSESSREG AITFTWVERS QNGGEPDFHA VEPYTKKELS AVTFPDIIRN YKVMAAENIP 660
ENPLKYLYPN IDKDHAFGKY YSRPKEAPEP MELDGPKGTG YIKTELISVS EV 712 
Gene Ontology
 GO:0030424; C:axon; ISS:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0030425; C:dendrite; ISS:UniProtKB.
 GO:0000790; C:nuclear chromatin; IDA:BHF-UCL.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0003690; F:double-stranded DNA binding; IDA:UniProtKB.
 GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
 GO:0000978; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding; IDA:BHF-UCL.
 GO:0000979; F:RNA polymerase II core promoter sequence-specific DNA binding; IDA:BHF-UCL.
 GO:0000983; F:RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity; IDA:BHF-UCL.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:UniProtKB.
 GO:0004871; F:signal transducer activity; IEA:InterPro.
 GO:0008015; P:blood circulation; ISS:UniProtKB.
 GO:0035458; P:cellular response to interferon-beta; IMP:BHF-UCL.
 GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
 GO:0006917; P:induction of apoptosis; ISS:UniProtKB.
 GO:0060333; P:interferon-gamma-mediated signaling pathway; IDA:BHF-UCL.
 GO:0060397; P:JAK-STAT cascade involved in growth hormone signaling pathway; TAS:Reactome.
 GO:0031663; P:lipopolysaccharide-mediated signaling pathway; IEA:Compara.
 GO:0072162; P:metanephric mesenchymal cell differentiation; ISS:UniProtKB.
 GO:0072136; P:metanephric mesenchymal cell proliferation involved in metanephros development; ISS:UniProtKB.
 GO:0016525; P:negative regulation of angiogenesis; IMP:UniProtKB.
 GO:0001937; P:negative regulation of endothelial cell proliferation; IMP:UniProtKB.
 GO:0043124; P:negative regulation of I-kappaB kinase/NF-kappaB cascade; IMP:UniProtKB.
 GO:0003340; P:negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis; ISS:UniProtKB.
 GO:0072308; P:negative regulation of metanephric nephron tubule epithelial cell differentiation; ISS:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; ISS:UniProtKB.
 GO:0002053; P:positive regulation of mesenchymal cell proliferation; ISS:UniProtKB.
 GO:0048661; P:positive regulation of smooth muscle cell proliferation; ISS:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0060334; P:regulation of interferon-gamma-mediated signaling pathway; TAS:Reactome.
 GO:0060338; P:regulation of type I interferon-mediated signaling pathway; TAS:Reactome.
 GO:0061326; P:renal tubule development; IMP:UniProtKB.
 GO:0051591; P:response to cAMP; ISS:UniProtKB.
 GO:0043330; P:response to exogenous dsRNA; IEA:Compara.
 GO:0033209; P:tumor necrosis factor-mediated signaling pathway; IDA:UniProtKB.
 GO:0060337; P:type I interferon-mediated signaling pathway; TAS:Reactome.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR008967; p53-like_TF_DNA-bd.
 IPR000980; SH2.
 IPR022752; STAT1_TAZ2-bd_C.
 IPR013800; STAT_TF_alpha.
 IPR015988; STAT_TF_coiled-coil.
 IPR001217; STAT_TF_core.
 IPR013801; STAT_TF_DNA-bd.
 IPR012345; STAT_TF_DNA-bd_sub.
 IPR013799; STAT_TF_prot_interaction. 
Pfam
 PF00017; SH2
 PF12162; STAT1_TAZ2bind
 PF01017; STAT_alpha
 PF02864; STAT_bind
 PF02865; STAT_int 
SMART
 SM00252; SH2
 SM00964; STAT_int 
PROSITE
 PS50001; SH2 
PRINTS