CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004128
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ribonuclease inhibitor 
Protein Synonyms/Alias
 Placental ribonuclease inhibitor; Placental RNase inhibitor; Ribonuclease/angiogenin inhibitor 1; RAI 
Gene Name
 RNH1 
Gene Synonyms/Alias
 PRI; RNH 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
46GLTEARCKDISSALRubiquitination[1, 2, 3]
86GLQTPSCKIQKLSLQubiquitination[3, 4]
89TPSCKIQKLSLQNCCubiquitination[1, 3, 4, 5, 6, 7]
173LRAKPDFKELTVSNNubiquitination[3, 5, 7]
195RVLCQGLKDSPCQLEubiquitination[3, 8]
205PCQLEALKLESCGVTubiquitination[3, 8, 9]
226LCGIVASKASLRELAubiquitination[1, 3, 4, 6]
238ELALGSNKLGDVGMAubiquitination[3]
283LCRVLRAKESLKELSubiquitination[3, 4]
287LRAKESLKELSLAGNubiquitination[1, 2, 3, 4, 6, 10]
452DRLQALEKDKPSLRVubiquitination[3, 4, 5, 7, 9]
454LQALEKDKPSLRVISubiquitination[5, 7, 9]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [8] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [9] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [10] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
 Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and ANG. May play a role in redox homeostasis. 
Sequence Annotation
 REPEAT 20 48 LRR 1.
 REPEAT 49 76 LRR 2.
 REPEAT 77 105 LRR 3.
 REPEAT 106 133 LRR 4.
 REPEAT 134 162 LRR 5.
 REPEAT 163 190 LRR 6.
 REPEAT 191 219 LRR 7.
 REPEAT 220 247 LRR 8.
 REPEAT 248 276 LRR 9.
 REPEAT 277 304 LRR 10.
 REPEAT 305 333 LRR 11.
 REPEAT 334 361 LRR 12.
 REPEAT 362 390 LRR 13.
 REPEAT 391 418 LRR 14.
 REPEAT 419 447 LRR 15.
 REGION 2 11 2 X 5 AA tandem repeats of S-L-D-I-Q.
 MOD_RES 82 82 Phosphothreonine.
 MOD_RES 91 91 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Leucine-rich repeat; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 461 AA 
Protein Sequence
MSLDIQSLDI QCEELSDARW AELLPLLQQC QVVRLDDCGL TEARCKDISS ALRVNPALAE 60
LNLRSNELGD VGVHCVLQGL QTPSCKIQKL SLQNCCLTGA GCGVLSSTLR TLPTLQELHL 120
SDNLLGDAGL QLLCEGLLDP QCRLEKLQLE YCSLSAASCE PLASVLRAKP DFKELTVSNN 180
DINEAGVRVL CQGLKDSPCQ LEALKLESCG VTSDNCRDLC GIVASKASLR ELALGSNKLG 240
DVGMAELCPG LLHPSSRLRT LWIWECGITA KGCGDLCRVL RAKESLKELS LAGNELGDEG 300
ARLLCETLLE PGCQLESLWV KSCSFTAACC SHFSSVLAQN RFLLELQISN NRLEDAGVRE 360
LCQGLGQPGS VLRVLWLADC DVSDSSCSSL AATLLANHSL RELDLSNNCL GDAGILQLVE 420
SVRQPGCLLE QLVLYDIYWS EEMEDRLQAL EKDKPSLRVI S 461 
Gene Ontology
 GO:0032311; C:angiogenin-PRI complex; IPI:UniProtKB.
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0008428; F:ribonuclease inhibitor activity; IDA:UniProtKB.
 GO:0006402; P:mRNA catabolic process; NAS:UniProtKB.
 GO:0045765; P:regulation of angiogenesis; IDA:UniProtKB. 
Interpro
 IPR003590; Leu-rich_rpt_RNase_inh_sub-typ. 
Pfam
  
SMART
 SM00368; LRR_RI 
PROSITE
  
PRINTS