CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022626
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Alpha-actinin-4 
Protein Synonyms/Alias
 F-actin cross-linking protein; Non-muscle alpha-actinin 4 
Gene Name
 Actn4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
114HKINNVNKALDFIASacetylation[1]
214PELIEYDKLRKDDPVacetylation[1]
417RLDHLAEKFRQKASIacetylation[1]
421LAEKFRQKASIHEAWubiquitination[2]
432HEAWTDGKEAMLKHRacetylation[1]
437DGKEAMLKHRDYETAacetylation[1]
518SRREALEKTEKQLETacetylation[1]
535QLHLEYAKRAAPFNNacetylation[1]
592EAILAIHKEAQRIAEacetylation[1]
622TPQIINSKWEKVQQLacetylation[1]
625IINSKWEKVQQLVPKacetylation[1]
632KVQQLVPKRDHALLEacetylation[1]
779ASFNHFDKDHGGALGacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (By similarity). 
Sequence Annotation
 DOMAIN 1 269 Actin-binding.
 DOMAIN 50 154 CH 1.
 DOMAIN 163 269 CH 2.
 REPEAT 293 403 Spectrin 1.
 REPEAT 413 518 Spectrin 2.
 REPEAT 528 639 Spectrin 3.
 REPEAT 649 752 Spectrin 4.
 DOMAIN 765 800 EF-hand 1.
 DOMAIN 806 841 EF-hand 2.
 REGION 177 192 Polyphosphoinositide (PIP2)-binding
 MOD_RES 114 114 N6-acetyllysine (By similarity).
 MOD_RES 592 592 N6-acetyllysine (By similarity).
 MOD_RES 625 625 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Actin-binding; Calcium; Complete proteome; Cytoplasm; Metal-binding; Nucleus; Protein transport; Reference proteome; Repeat; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 911 AA 
Protein Sequence
MVDYHAANQA YQYGPSSGGN GTGGGGGMGD YMAQEDDWDR DLLLDPAWEK QQRKTFTAWC 60
NSHLRKAGTQ IENIDEDFRD GLKLMLLLEV ISGERLPKPE RGKMRVHKIN NVNKALDFIA 120
SKGVKLVSIG AEEIVDGNAK MTLGMIWTII LRFAIQDISV EETSAKEGLL LWCQRKTAPY 180
KNVNVQNFHI SWKDGLAFNA LIHRHRPELI EYDKLRKDDP VTNLNNAFEV AEKYLDIPKM 240
LDAEDIVNTA RPDEKAIMTY VSSFYHAFSG AQKAETAANR ICKVLAVNQE NEHLMEDYER 300
LASDLLEWIR RTIPWLEDRV PQKTIQEMQQ KLEDFRDYRR VHKPPKVQEK CQLEINFNTL 360
QTKLRLSNRP AFMPSEGRMV SDINNGWQHL EQAEKGYEEW LLNEIRRLER LDHLAEKFRQ 420
KASIHEAWTD GKEAMLKHRD YETATLSDIK ALIRKHEAFE SDLAAHQDRV EQIAAIAQEL 480
NELDYYDSHN VNTRCQKICD QWDNLGSLTH SRREALEKTE KQLETIDQLH LEYAKRAAPF 540
NNWMESAMED LQDMFIVHTI EEIEGLISAH DQFKSTLPDA DREREAILAI HKEAQRIAES 600
NHIKLSGSNP YTSVTPQIIN SKWEKVQQLV PKRDHALLEE QSKQQSNEHL RRQFASQANM 660
VGPWIQTKME EIGRISIEMN GTLEDQLSHL KQYERSIVDY KPNLDLLEQQ HQLIQEALIF 720
DNKHTNYTME HLRVGWEQLL TTIARTINEV ENQILTRDAK GISQEQMQEF RASFNHFDKD 780
HGGALGPEEF KACLISLGYD VENDRQGDAE FNRIMSVVDP NHSGLVTFQA FIDFMSRETT 840
DTDTADQVIA SFKVLAGDKN FITAEELRRE LPPDQAEYCI ARMAPYQGPD AAPGALDYKS 900
FSTALYGESD L 911 
Gene Ontology
 GO:0030863; C:cortical cytoskeleton; IEA:Compara.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0070062; C:extracellular vesicular exosome; IEA:Compara.
 GO:0005634; C:nucleus; IDA:RGD.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:Compara.
 GO:0043234; C:protein complex; IDA:RGD.
 GO:0031143; C:pseudopodium; IEA:Compara.
 GO:0030529; C:ribonucleoprotein complex; ISS:UniProtKB.
 GO:0030018; C:Z disc; IEA:Compara.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0001882; F:nucleoside binding; IEA:Compara.
 GO:0051764; P:actin crosslink formation; IEA:InterPro.
 GO:0051017; P:actin filament bundle assembly; IEA:Compara.
 GO:0051271; P:negative regulation of cellular component movement; IEA:Compara.
 GO:0051272; P:positive regulation of cellular component movement; IEA:Compara.
 GO:0048549; P:positive regulation of pinocytosis; IEA:Compara.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW.
 GO:0042981; P:regulation of apoptotic process; IMP:RGD.
 GO:0001666; P:response to hypoxia; IEP:RGD. 
Interpro
 IPR001589; Actinin_actin-bd_CS.
 IPR026921; Alpha-actinin.
 IPR001715; CH-domain.
 IPR011992; EF-hand-like_dom.
 IPR014837; EF-hand_Ca_insen.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR018159; Spectrin/alpha-actinin.
 IPR002017; Spectrin_repeat. 
Pfam
 PF00307; CH
 PF13405; EF_hand_4
 PF08726; efhand_Ca_insen
 PF00435; Spectrin 
SMART
 SM00033; CH
 SM00054; EFh
 SM00150; SPEC 
PROSITE
 PS00019; ACTININ_1
 PS00020; ACTININ_2
 PS50021; CH
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2 
PRINTS