UniProt Accession

 IF4A2_HUMAN; Q14240; D3DNU9; Q53XJ6; Q96B90; Q96EA8 

Genbank Protein ID

 D30655; BT009860; AC112907; CH471052; CH471052; BC012547; BC013708; BC015842; BC048105 

Genbank Nucleotide ID

 BAA06336.1; AAP88862.1; EAW78172.1; EAW78177.1; AAH12547.1; AAH13708.1; AAH15842.1; AAH48105.1 

Protein Name

 Eukaryotic initiation factor 4A-II 

Protein Synonyms/Alias

 eIF-4A-II; eIF4A-II; ATP-dependent RNA helicase eIF4A-2 

Gene Name

 EIF4A2 

Gene Synonyms/Alias

 DDX2B; EIF4F 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
69	RAIIPCIKGYDVIAQ	ubiquitination	[1, 2, 3]
147	NVRNEMQKLQAEAPH	ubiquitination	[2]
175	NRRYLSPKWIKMFVL	ubiquitination	[4]
194	EMLSRGFKDQIYEIF	ubiquitination	[2]
310	LHGDMDQKERDVIMR	ubiquitination	[2]
370	RGGRFGRKGVAINFV	ubiquitination	[5, 6]
382	NFVTEEDKRILRDIE	ubiquitination	[5, 6, 7]

Reference

 [1] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
 Xu G, Paige JS, Jaffrey SR.
 Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. 

Sequence Annotation

 DOMAIN 64 235 Helicase ATP-binding.
 DOMAIN 246 407 Helicase C-terminal.
 NP_BIND 77 84 ATP (By similarity).
 MOTIF 33 61 Q motif.
 MOTIF 183 186 DEAD box.  

Keyword

 3D-structure; Alternative splicing; ATP-binding; Complete proteome; Helicase; Host-virus interaction; Hydrolase; Initiation factor; Nucleotide-binding; Polymorphism; Protein biosynthesis; Reference proteome; RNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 407 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0016281; C:eukaryotic translation initiation factor 4F complex; TAS:ProtInc.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0004386; F:helicase activity; TAS:ProtInc.
 GO:0003743; F:translation initiation factor activity; TAS:ProtInc.
 GO:0019221; P:cytokine-mediated signaling pathway; TAS:Reactome.
 GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; TAS:Reactome.
 GO:0006446; P:regulation of translational initiation; TAS:ProtInc.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 

Interpro

 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000629; RNA-helicase_DEAD-box_CS.
 IPR014014; RNA_helicase_DEAD_Q_motif. 

Pfam

 PF00270; DEAD
 PF00271; Helicase_C 

SMART

 SM00487; DEXDc
 SM00490; HELICc 

PROSITE

 PS00039; DEAD_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51195; Q_MOTIF 

PRINTS