CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007781
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Crk-like protein 
Protein Synonyms/Alias
  
Gene Name
 CRKL 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
180IPVPYVEKLVRSSPHubiquitination[1]
253RVPCAYDKTALALEVacetylation[2]
292LFPFTHVKIFDPQNPubiquitination[1, 3, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 May mediate the transduction of intracellular signals. 
Sequence Annotation
 DOMAIN 14 102 SH2.
 DOMAIN 123 183 SH3 1.
 DOMAIN 235 296 SH3 2.
 MOD_RES 107 107 Phosphoserine (By similarity).
 MOD_RES 127 127 Phosphotyrosine.
 MOD_RES 132 132 Phosphotyrosine (By similarity).
 MOD_RES 207 207 Phosphotyrosine.
 MOD_RES 251 251 Phosphotyrosine (By similarity).  
Keyword
 3D-structure; Complete proteome; Phosphoprotein; Reference proteome; Repeat; SH2 domain; SH3 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 303 AA 
Protein Sequence
MSSARFDSSD RSAWYMGPVS RQEAQTRLQG QRHGMFLVRD SSTCPGDYVL SVSENSRVSH 60
YIINSLPNRR FKIGDQEFDH LPALLEFYKI HYLDTTTLIE PAPRYPSPPM GSVSAPNLPT 120
AEDNLEYVRT LYDFPGNDAE DLPFKKGEIL VIIEKPEEQW WSARNKDGRV GMIPVPYVEK 180
LVRSSPHGKH GNRNSNSYGI PEPAHAYAQP QTTTPLPAVS GSPGAAITPL PSTQNGPVFA 240
KAIQKRVPCA YDKTALALEV GDIVKVTRMN INGQWEGEVN GRKGLFPFTH VKIFDPQNPD 300
ENE 303 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005768; C:endosome; TAS:Reactome.
 GO:0005070; F:SH3/SH2 adaptor activity; TAS:ProtInc.
 GO:0004871; F:signal transducer activity; TAS:ProtInc.
 GO:0000186; P:activation of MAPKK activity; TAS:Reactome.
 GO:0009952; P:anterior/posterior pattern specification; IEA:Compara.
 GO:0001568; P:blood vessel development; IEA:Compara.
 GO:0007507; P:heart development; IEA:Compara.
 GO:0007254; P:JNK cascade; TAS:ProtInc.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0009887; P:organ morphogenesis; IEA:Compara.
 GO:0060017; P:parathyroid gland development; IEA:Compara.
 GO:0007265; P:Ras protein signal transduction; TAS:ProtInc.
 GO:0048538; P:thymus development; IEA:Compara. 
Interpro
 IPR000980; SH2.
 IPR011511; SH3_2.
 IPR001452; SH3_domain. 
Pfam
 PF00017; SH2
 PF00018; SH3_1
 PF07653; SH3_2 
SMART
 SM00252; SH2
 SM00326; SH3 
PROSITE
 PS50001; SH2
 PS50002; SH3 
PRINTS
 PR00401; SH2DOMAIN.
 PR00452; SH3DOMAIN.