CPLM-022212

Genbank Nucleotide ID

ATPase family AAA domain-containing protein 3A

Protein Synonyms/Alias

Homo sapiens (Human)

Position	Peptide	Type	References
183	ARAQYQDKLARQRYE	ubiquitination	[1]
406	YGPPGTGKTLFAKKL	ubiquitination	[1, 2, 3]
442	EGVTAMHKLFDWANT	ubiquitination	[2, 3]
472	LRKRATEKISEDLRA	ubiquitination	[1]
539	LVRMYFDKYVLKPAT	acetylation	[4]
543	YFDKYVLKPATEGKQ	acetylation	[4, 5]
549	LKPATEGKQRLKLAQ	ubiquitination	[1]
621	QQKMCWLKAEGPGRG	ubiquitination	[2, 3]

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
[5] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330]

Functional Description

Essential for mitochondrial network organization, mitochondrial metabolism and cell growth at organism and cellular level. May play an important in mitochondrial protein synthesis. May also participate in mitochondrial DNA replication. May bind to mitochondrial DNA D-loops and contribute to nucleoid stability. Required for enhanced channeling of cholesterol for hormone- dependent steroidogenesis.

NP_BIND 400 407 ATP (Potential).
REGION 2 50 Required for interaction with the inner
REGION 338 353 S100B-binding.
MOD_RES 2 2 N-acetylserine.
MOD_RES 539 539 N6-acetyllysine.

Acetylation; Alternative splicing; ATP-binding; Coiled coil; Complete proteome; Direct protein sequencing; Membrane; Mitochondrion; Mitochondrion inner membrane; Mitochondrion nucleoid; Nucleotide-binding; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
GO:0042645; C:mitochondrial nucleoid; IEA:UniProtKB-SubCell.
GO:0005739; C:mitochondrion; IDA:UniProtKB.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO:0017111; F:nucleoside-triphosphatase activity; IEA:InterPro.
GO:0016049; P:cell growth; IMP:UniProtKB.
GO:0043066; P:negative regulation of apoptotic process; IMP:UniProtKB.

IPR003593; AAA+_ATPase.
IPR003959; ATPase_AAA_core.
IPR021911; DUF3523.
IPR027417; P-loop_NTPase.

PF00004; AAA
PF12037; DUF3523