UniProt Accession

 NFL_RAT; P19527; Q63367 

Genbank Protein ID

 AF031880; M25638; X53981 

Genbank Nucleotide ID

 AAB87069.1; AAA41694.1; CAA37931.1 

Protein Name

 Neurofilament light polypeptide 

Protein Synonyms/Alias

 NF-L; 68 kDa neurofilament protein; Neurofilament triplet L protein 

Gene Name

 Nefl 

Gene Synonyms/Alias

 Nf68; Nfl 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
15	PYFSTSYKRRYVETP	acetylation	[1]
91	KSIRTQEKAQLQDLN	acetylation	[1]
157	AEDATNEKQALQGER	acetylation	[1]
224	MDEIAFLKKVHEEEI	acetylation	[1]
268	DIRAQYEKLAAKNMQ	acetylation	[1]
268	DIRAQYEKLAAKNMQ	ubiquitination	[2]
282	QNAEEWFKSRFTVLT	acetylation	[1]
294	VLTESAAKNTDAVRA	acetylation	[1]
303	TDAVRAAKDEVSESR	acetylation	[1]
340	QLQELEDKQNADISA	acetylation	[1]
354	AMQDTINKLENELRS	acetylation	[1]
363	ENELRSTKSEMARYL	acetylation	[1]
371	SEMARYLKEYQDLLN	acetylation	[1]
392	IEIAAYRKLLEGEET	ubiquitination	[2]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 

Functional Description

 Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. 

Sequence Annotation

 REGION 2 93 Head.
 REGION 94 397 Rod.
 REGION 94 125 Coil 1A.
 REGION 126 138 Linker 1.
 REGION 139 234 Coil 1B.
 REGION 235 253 Linker 12.
 REGION 254 272 Coil 2A.
 REGION 273 281 Linker 2.
 REGION 282 397 Coil 2B.
 REGION 382 392 Epitope; recognized by IF-specific
 REGION 398 542 Tail.
 REGION 398 444 Tail, subdomain A.
 REGION 445 542 Tail, subdomain B (acidic).
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 43 43 Phosphotyrosine (By similarity).
 MOD_RES 56 56 Phosphoserine (By similarity).
 MOD_RES 67 67 Phosphoserine (By similarity).
 MOD_RES 425 425 Phosphotyrosine (By similarity).
 MOD_RES 433 433 Phosphotyrosine (By similarity).
 MOD_RES 473 473 Phosphoserine (By similarity).
 MOD_RES 531 531 Phosphoserine (By similarity).
 CARBOHYD 21 21 O-linked (GlcNAc).
 CARBOHYD 27 27 O-linked (GlcNAc).  

Keyword

 Acetylation; Coiled coil; Complete proteome; Direct protein sequencing; Glycoprotein; Intermediate filament; Phosphoprotein; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 542 AA 

Protein Sequence

Gene Ontology

 GO:0030424; C:axon; IDA:RGD.
 GO:0030426; C:growth cone; IDA:RGD.
 GO:0005883; C:neurofilament; IEA:Compara.
 GO:0005198; F:structural molecule activity; IEA:InterPro.
 GO:0045110; P:intermediate filament bundle assembly; IEA:Compara.
 GO:0040011; P:locomotion; IEA:Compara.
 GO:0000226; P:microtubule cytoskeleton organization; IEA:Compara.
 GO:0043524; P:negative regulation of neuron apoptotic process; IEA:Compara.
 GO:0060052; P:neurofilament cytoskeleton organization; IEA:Compara.
 GO:0050885; P:neuromuscular process controlling balance; IEA:Compara.
 GO:0014012; P:peripheral nervous system axon regeneration; IEA:Compara.
 GO:0050772; P:positive regulation of axonogenesis; IEA:Compara.
 GO:0051258; P:protein polymerization; IDA:RGD.
 GO:0031133; P:regulation of axon diameter; IEA:Compara.
 GO:0051412; P:response to corticosterone stimulus; IEP:RGD.
 GO:0043434; P:response to peptide hormone stimulus; IEP:RGD.
 GO:0009636; P:response to toxic substance; IEP:RGD. 

Interpro

 IPR016044; F.
 IPR001664; IF.
 IPR006821; Intermed_filament_DNA-bd.
 IPR018039; Intermediate_filament_CS. 

Pfam

 PF00038; Filament
 PF04732; Filament_head 

SMART

  

PROSITE

 PS00226; IF 

PRINTS