CPLM-018503

CPLM 1.0 - Compendium of Protein Lysine Modification

Tag

Content

CPLM ID

CPLM-018503

UniProt Accession

CDKAL_MOUSE; Q91WE6; Q3TE97; Q3V3D2; Q5T0I5; Q8C4B0; Q9CT69

Genbank Protein ID

AK004490; AK041864; AK045506; AK082629; AK169761; AL513025; AL512647; AL589701; AL645587; AL512647; AL513025; AL589701; AL645587; AL645587; AL512647; AL513025; AL589701; AL589701; AL512647; AL513025; AL645587; AL513025; BC016073

Genbank Nucleotide ID

BAB23330.1; BAE20608.1; BAE43325.1; BAC38554.1; BAE41351.1; CAI24677.1; CAI24677.1; CAI24677.1; CAI24677.1; CAI25816.1; CAI25816.1; CAI25816.1; CAI25816.1; CAI35144.1; CAI35144.1; CAI35144.1; CAI35144.1; CAI35240.1; CAI35240.1; CAI35240.1; CAI35240.1; CAI24675.1; AAH16073.1

Protein Name

Threonylcarbamoyladenosine tRNA methylthiotransferase

Protein Synonyms/Alias

CDK5 regulatory subunit-associated protein 1-like 1; tRNA-t(6)A37 methylthiotransferase

Gene Name

Cdkal1

Gene Synonyms/Alias

Created Date

July 27, 2013

Organism

Mus musculus (Mouse)

NCBI Taxa ID

10090

Lysine Modification

Position	Peptide	Type	References
515	GEVSGLTKEFRNRLG	ubiquitination	[1]

Reference

[1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]

Functional Description

Catalyzes the methylthiolation of N6- threonylcarbamoyladenosine (t(6)A), leading to the formation of 2- methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine (By similarity).

Sequence Annotation

DOMAIN 63 171 MTTase N-terminal.
DOMAIN 430 492 TRAM.
METAL 72 72 Iron-sulfur (4Fe-4S) (By similarity).
METAL 108 108 Iron-sulfur (4Fe-4S) (By similarity).
METAL 137 137 Iron-sulfur (4Fe-4S) (By similarity).
METAL 213 213 Iron-sulfur (4Fe-4S-S-AdoMet) (By
METAL 217 217 Iron-sulfur (4Fe-4S-S-AdoMet) (By
METAL 220 220 Iron-sulfur (4Fe-4S-S-AdoMet) (By
MOD_RES 498 498 Phosphothreonine (By similarity).

Keyword

4Fe-4S; Alternative splicing; Complete proteome; Endoplasmic reticulum; Iron; Iron-sulfur; Membrane; Metal-binding; Phosphoprotein; Reference proteome; S-adenosyl-L-methionine; Transferase; Transmembrane; Transmembrane helix; tRNA processing.

Sequence Source

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

Protein Length

578 AA

Protein Sequence

MPSASCDVLL DDIEDIISQE DSKPQDRQFS RKHVFPKVRR RNTQKYLQEE PRPPSDSTIP 60
GIQKIWIRTW GCSHNNSDGE YMAGQLAAYG YKITENASDA DLWLLNSCTV KNPAEDHFRN 120
SIKKAQEENK KVVLAGCVPQ AQPRQDYLKG LSIIGVQQID RVVEVVEETI KGHSVRLLGQ 180
KKDNGKRLGG ARLDLPKIRK NPLIEIISIN TGCLNACTYC KTKHARGNLA SYPIDELVER 240
AKQSFQEGVC EIWLTSEDTG AYGRDIGTDL PTLLWKLVEV IPEGAMLRLG MTNPPYILEH 300
LEEMAKILNH PRVYAFLHIP VQSASDSVLM DMKREYCVAD FKRVVDFLKE KVPGITIATD 360
IICGFPGETD QDFQETVKLV EEYKFPSLFI NQFYPRPGTP AAKAEQVPAH VKKQRTKDLS 420
RVFHSYNPYD HKIGERQQVL VTEESFDSKF YVAHNRFYEQ VLVPKNPAFM GKMVEVDIYE 480
SGKHFLKGQP VSETRVYTPS ISKPLAKGEV SGLTKEFRNR LGNHPNGTSD TCPATQHGSA 540
YSRMVLQMSQ YDCALKVATG LALLALLLHF WPDSLLTM 578

Gene Ontology

GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
GO:0009451; P:RNA modification; IEA:InterPro.
GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.

Interpro

IPR006638; Elp3/MiaB/NifB.
IPR023970; MeThioTfrase/rSAM.
IPR005839; Methylthiotransferase.
IPR020612; Methylthiotransferase_CS.
IPR013848; Methylthiotransferase_N.
IPR006466; MiaB-like_B.
IPR007197; rSAM.
IPR023404; rSAM_horseshoe.
IPR002792; TRAM_dom.

Pfam

PF04055; Radical_SAM
PF01938; TRAM
PF00919; UPF0004

SMART

SM00729; Elp3

PROSITE

PS51449; MTTASE_N
PS01278; MTTASE_RADICAL
PS50926; TRAM

PRINTS