CPLM-020476

Genbank Nucleotide ID

Lactoylglutathione lyase

Protein Synonyms/Alias

Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase

Mus musculus (Mouse)

Position	Peptide	Type	References
44	LRIKDPKKSLDFYTR	acetylation	[1]
60	LGLTLLQKLDFPAMK	acetylation	[1]
60	LGLTLLQKLDFPAMK	ubiquitination	[2]
88	IPKDKSEKTAWTFSR	acetylation	[1, 3]
88	IPKDKSEKTAWTFSR	succinylation	[3]
88	IPKDKSEKTAWTFSR	ubiquitination	[2]
96	TAWTFSRKATLELTH	ubiquitination	[2]
140	PDVYSACKRFEELGV	acetylation	[1, 4]
140	PDVYSACKRFEELGV	ubiquitination	[2]
148	RFEELGVKFVKKPDD	acetylation	[1, 3, 4, 5]
148	RFEELGVKFVKKPDD	succinylation	[3]
148	RFEELGVKFVKKPDD	ubiquitination	[2]
151	ELGVKFVKKPDDGKM	acetylation	[1, 4]
152	LGVKFVKKPDDGKMK	acetylation	[1]

[1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
[2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
[3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
[4] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
[5] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
Simon GM, Cheng J, Gordon JI.
Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]

Functional Description

Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF- kappa-B (By similarity).

METAL 34 34 Zinc (By similarity).
METAL 100 100 Zinc (By similarity).
METAL 127 127 Zinc (By similarity).
METAL 173 173 Zinc (By similarity).
MOD_RES 2 2 N-acetylalanine (By similarity).
MOD_RES 107 107 Phosphothreonine (By similarity).
MOD_RES 139 139 S-glutathionyl cysteine (By similarity).
MOD_RES 148 148 N6-acetyllysine (By similarity).
DISULFID 19 20 By similarity.

3D-structure; Acetylation; Complete proteome; Disulfide bond; Glutathionylation; Lyase; Metal-binding; Phosphoprotein; Reference proteome; Zinc.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0004462; F:lactoylglutathione lyase activity; ISS:UniProtKB.
GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO:0005975; P:carbohydrate metabolic process; IEA:Compara.
GO:0006749; P:glutathione metabolic process; IEA:Compara.
GO:0009438; P:methylglyoxal metabolic process; IEA:Compara.
GO:0043066; P:negative regulation of apoptotic process; ISS:UniProtKB.
GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IDA:MGI.

IPR004360; Glyas_Fos-R_dOase_dom.
IPR004361; Glyoxalase_1.
IPR018146; Glyoxalase_1_CS.

PS00934; GLYOXALASE_I_1
PS00935; GLYOXALASE_I_2