UniProt Accession

 HAUS3_HUMAN; Q68CZ6; O43606; Q8TAZ5; Q9BTJ9 

Genbank Protein ID

 AF040964; CR749640; CH471131; BC003648; BC025356 

Genbank Nucleotide ID

 AAB97010.1; CAH18434.1; EAW82537.1; AAH03648.1; AAH25356.1 

Protein Name

 HAUS augmin-like complex subunit 3 

Protein Synonyms/Alias

  

Gene Name

 HAUS3 

Gene Synonyms/Alias

 C4orf15 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
12	NEFVETLKKIGYPKA	ubiquitination	[1]
66	EAFSILQKSGKPILE	ubiquitination	[1]
69	SILQKSGKPILEGAA	ubiquitination	[1]
111	DEVQTLLKLKNLKIQ	ubiquitination	[2]
219	AALTLYTKKQFFQGI	ubiquitination	[2, 3]
291	SSMKSSIKWAEESLH	ubiquitination	[1]
303	SLHSLTSKAVDKENL	ubiquitination	[2]
313	DKENLDAKISSLTSE	ubiquitination	[2]
323	SLTSEIMKLEKEVTQ	ubiquitination	[1]
326	SEIMKLEKEVTQIKD	ubiquitination	[1]
332	EKEVTQIKDRSLPAV	ubiquitination	[1]
362	DFDLQIAKQDYYTAR	ubiquitination	[2, 3]
379	LVLNQLIKQKASFEL	ubiquitination	[1, 2, 4, 5]
444	PRNTIDTKDYSTHRL	ubiquitination	[1]
462	LEGENKKKELFLTHG	ubiquitination	[1]
479	EEVAEKLKQNISLVQ	ubiquitination	[1]
502	EHSFFLSKRNKDVDM	ubiquitination	[1]
554	TDILADVKTKRKTLA	ubiquitination	[1, 2]
564	RKTLANNKLHQMERE	ubiquitination	[1, 2, 4, 5, 6]
584	LKDEDYLKDIVENLE	ubiquitination	[2]
597	LETQSKIKAVSLED*	ubiquitination	[1, 2, 6]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. 

Sequence Annotation

 MOD_RES 2 2 N-acetylserine.  

Keyword

 Acetylation; Cell cycle; Cell division; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Microtubule; Mitosis; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 603 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0070652; C:HAUS complex; IDA:UniProtKB.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0051297; P:centrosome organization; IMP:UniProtKB.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0051225; P:spindle assembly; IMP:UniProtKB. 

Interpro

 IPR026206; HAUS3. 

Pfam

  

SMART

  

PROSITE

  

PRINTS

 PR02089; HAUSAUGMINL3.