UniProt Accession

 SYK2_ECOLI; P0A8N5; P14825; Q2M6H4 

Genbank Protein ID

 X16542; M30630; U14003; U00096; AP009048 

Genbank Nucleotide ID

 CAA34542.1; AAA24096.1; AAA97029.1; AAC77090.1; BAE78132.1 

Protein Name

 Lysine--tRNA ligase, heat inducible 

Protein Synonyms/Alias

 Lysyl-tRNA synthetase; LysRS 

Gene Name

 lysU 

Gene Synonyms/Alias

 b4129; JW4090 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
114	GVYNDQFKKWDLGDI	acetylation	[1, 2, 3]
115	VYNDQFKKWDLGDII	acetylation	[2, 3]
156	ALRPLPDKFHGLQDQ	acetylation	[1, 2, 3]
179	LDLIANDKSRQTFVV	acetylation	[3]
310	QEVLGTTKVTYGEHV	acetylation	[2, 3]
322	EHVFDFGKPFEKLTM	acetylation	[2, 3]
335	TMREAIKKYRPETDM	acetylation	[2]

Reference

 [1] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
 Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y.
 Mol Cell Proteomics. 2009 Feb;8(2):215-25. [PMID: 18723842]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [3] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Also can synthesize a number of adenyl dinucleotides (in particular AppppA). These dinucleotides have been proposed to act as modulators of the heat-shock response and stress response. 

Sequence Annotation

 METAL 415 415 Magnesium 1.
 METAL 422 422 Magnesium 1.
 METAL 422 422 Magnesium 2.
 MOD_RES 114 114 N6-acetyllysine.
 MOD_RES 156 156 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Ligase; Magnesium; Metal-binding; Nucleotide-binding; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 505 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0016020; C:membrane; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IDA:EcoliWiki.
 GO:0004824; F:lysine-tRNA ligase activity; IDA:EcoCyc.
 GO:0000287; F:magnesium ion binding; IDA:EcoliWiki.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0006430; P:lysyl-tRNA aminoacylation; IDA:EcoCyc. 

Interpro

 IPR004364; aa-tRNA-synt_II.
 IPR018150; aa-tRNA-synt_II-like.
 IPR006195; aa-tRNA-synth_II.
 IPR002313; Lys-tRNA-ligase_II.
 IPR018149; Lys-tRNA-synth_II_C.
 IPR012340; NA-bd_OB-fold.
 IPR004365; NA-bd_OB_tRNA-helicase. 

Pfam

 PF00152; tRNA-synt_2
 PF01336; tRNA_anti 

SMART

  

PROSITE

 PS50862; AA_TRNA_LIGASE_II 

PRINTS

 PR00982; TRNASYNTHLYS.