UniProt Accession

 RGPD3_HUMAN; A6NKT7; B8ZZM4 

Genbank Protein ID

 AC097527; AC114755 

Genbank Nucleotide ID

  

Protein Name

 RanBP2-like and GRIP domain-containing protein 3 

Protein Synonyms/Alias

  

Gene Name

 RGPD3 

Gene Synonyms/Alias

 RGP3 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
94	VELNPTQKDLVLKIA	ubiquitination	[1, 2, 3]
106	KIAELLCKNDVTDGR	ubiquitination	[2, 4]
123	YWVERAAKLFPGSPA	ubiquitination	[2, 4, 5]
133	PGSPAIYKLKEQLLD	ubiquitination	[2]
135	SPAIYKLKEQLLDCE	ubiquitination	[2, 6]
336	RPKIKLIKGEAGQNL	ubiquitination	[2]
380	VVETFANKSGQSALY	ubiquitination	[3]
608	GRSVHYWKKVLPLLK	ubiquitination	[2]
609	RSVHYWKKVLPLLKI	ubiquitination	[2]
615	KKVLPLLKIIKKKNS	ubiquitination	[5]
619	PLLKIIKKKNSIPEP	ubiquitination	[2]
620	LLKIIKKKNSIPEPI	ubiquitination	[2, 4]
734	DSNLSVVKKLPVPLE	ubiquitination	[3, 4, 5, 7]
735	SNLSVVKKLPVPLES	ubiquitination	[2]
785	HSTPSPTKYSLSPSK	ubiquitination	[2]
792	KYSLSPSKSYKYSPK	ubiquitination	[2]
799	KSYKYSPKTPPRWAE	ubiquitination	[2, 4]
924	PVSADGFKFGISEPG	ubiquitination	[2]
935	SEPGNQEKKSEKPLE	ubiquitination	[2]
1002	GFSGAGEKLFSSQYG	ubiquitination	[8]
1058	VTGEEGEKVLYSQGV	ubiquitination	[2]
1066	VLYSQGVKLFRFDAE	ubiquitination	[2, 3]
1086	ERGLGNLKILKNEVN	ubiquitination	[2, 5, 8, 9]
1089	LGNLKILKNEVNGKV	ubiquitination	[2]
1121	ITTTMNLKPLSGSDR	ubiquitination	[2]
1161	PELAEEFKQKFEECQ	ubiquitination	[1, 2]
1163	LAEEFKQKFEECQRL	ubiquitination	[2]
1181	IPLQTPHKLVDTGRA	ubiquitination	[2, 9]
1199	IQRAEEMKSGLKDFK	ubiquitination	[2]
1203	EEMKSGLKDFKTFLT	ubiquitination	[2, 4]
1206	KSGLKDFKTFLTNDQ	ubiquitination	[2, 5]
1304	KSALSLSKSPAKLNQ	ubiquitination	[2]
1369	AEFYRYDKDVGQWKE	ubiquitination	[2]
1375	DKDVGQWKERGIGDI	ubiquitination	[2]
1383	ERGIGDIKILQNYDN	ubiquitination	[2, 5, 8]
1404	MRRDQVLKLCANHRI	ubiquitination	[1, 2]
1421	DMSLQNMKGTERVWV	ubiquitination	[1, 2, 4]
1449	EHLAVRFKLQDVADS	ubiquitination	[2, 5, 8]
1459	DVADSFKKIFDEAKT	ubiquitination	[2]
1465	KKIFDEAKTAQEKDS	ubiquitination	[2]
1470	EAKTAQEKDSLITPH	ubiquitination	[2]
1491	PRESPCGKIAVAVLE	ubiquitination	[2]
1547	VFGSESVKRIFSSEK	ubiquitination	[2]
1596	AQSGSESKVEPKKCE	ubiquitination	[1]
1606	PKKCELSKNSDIEQS	ubiquitination	[2]
1617	IEQSSDSKVKNLSAS	ubiquitination	[2]
1691	MEQIKLLKSEIRRLE	ubiquitination	[2]
1714	AANVEHLKNVLLQFI	ubiquitination	[2]
1724	LLQFIFLKPGSERER	ubiquitination	[2]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [8] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [9] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

  

Sequence Annotation

 REPEAT 60 93 TPR 1.
 REPEAT 584 617 TPR 2.
 DOMAIN 1037 1173 RanBD1 1.
 DOMAIN 1334 1470 RanBD1 2.
 DOMAIN 1703 1753 GRIP.
 MOD_RES 21 21 Phosphoserine (By similarity).  

Keyword

 Coiled coil; Complete proteome; Phosphoprotein; Reference proteome; Repeat; TPR repeat. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1758 AA 

Protein Sequence

Gene Ontology

 GO:0000042; P:protein targeting to Golgi; IEA:InterPro. 

Interpro

 IPR000237; GRIP.
 IPR011993; PH_like_dom.
 IPR000156; Ran_bind_dom.
 IPR001440; TPR-1.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR019734; TPR_repeat. 

Pfam

 PF01465; GRIP
 PF00638; Ran_BP1
 PF00515; TPR_1 

SMART

 SM00755; Grip
 SM00160; RanBD 

PROSITE

 PS50913; GRIP
 PS50196; RANBD1
 PS50005; TPR
 PS50293; TPR_REGION 

PRINTS