CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029764
UniProt Accession
Genbank Protein ID
 Z93241 
Genbank Nucleotide ID
  
Protein Name
 NADH-cytochrome b5 reductase 3 membrane-bound form 
Protein Synonyms/Alias
  
Gene Name
 CYB5R3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
19TLESPDIKYPLRLIDubiquitination[1, 2, 3, 4, 5]
92LVIKVYFKDTHPKFPubiquitination[4]
97YFKDTHPKFPAGGKMubiquitination[2, 3, 4, 5]
103PKFPAGGKMSQYLESubiquitination[2, 3, 4]
133LVYQGKGKFAIRPDKubiquitination[6]
140KFAIRPDKKSNPIIRubiquitination[2, 3, 4, 5, 6]
141FAIRPDKKSNPIIR*ubiquitination[5]
Reference
 [1] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 147 AA 
Protein Sequence
MKLFQRSTPA ITLESPDIKY PLRLIDREII SHDTRRFRFA LPSPQHILGL PVGQHIYLSA 60
RIDGNLVVRP YTPISSDDDK GFVDLVIKVY FKDTHPKFPA GGKMSQYLES MQIGDTIEFR 120
GPSGLLVYQG KGKFAIRPDK KSNPIIR 147 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IEA:Compara.
 GO:0005743; C:mitochondrial inner membrane; IEA:Compara.
 GO:0043531; F:ADP binding; IEA:Compara.
 GO:0016208; F:AMP binding; IEA:Compara.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:Compara.
 GO:0051287; F:NAD binding; IEA:Compara.
 GO:0016491; F:oxidoreductase activity; IEA:InterPro. 
Interpro
 IPR017927; Fd_Rdtase_FAD-bd.
 IPR001834; NADH-Cyt_B5_reductase.
 IPR008333; OxRdtase_FAD-bd_dom.
 IPR017938; Riboflavin_synthase-like_b-brl. 
Pfam
 PF00970; FAD_binding_6 
SMART
  
PROSITE
 PS51384; FAD_FR 
PRINTS
 PR00406; CYTB5RDTASE.