CPLM-024033

CPLM 1.0 - Compendium of Protein Lysine Modification

Tag

Content

CPLM ID

CPLM-024033

UniProt Accession

M3K4_HUMAN; Q9Y6R4; A6H8W0; B7ZLD3; B9EG75; Q5VTT8; Q5VTT9; Q92612; Q9H408

Genbank Protein ID

AF002715; D86968; AL109942; AL139393; AL591045; AL596452; AL109942; AL139393; AL591045; AL596452; AL139393; AL109942; AL591045; AL596452; AL139393; AL109942; AL591045; AL596452; AL591045; AL109942; AL139393; AL596452; AL591045; AL109942; AL139393; AL596452; AL596452; AL109942; AL139393; AL591045; AL596452; AL109942; AL139393; AL591045; CH471051; BC136276; BC143735; BC146770

Genbank Nucleotide ID

AAB68804.1; BAA13204.2; CAC12766.2; CAC12766.2; CAC12766.2; CAC12766.2; CAI21477.1; CAI21477.1; CAI21477.1; CAI21477.1; CAI20815.1; CAI20815.1; CAI20815.1; CAI20815.1; CAI20816.1; CAI20816.1; CAI20816.1; CAI20816.1; CAH70639.1; CAH70639.1; CAH70639.1; CAH70639.1; CAH70640.1; CAH70640.1; CAH70640.1; CAH70640.1; CAI41309.1; CAI41309.1; CAI41309.1; CAI41309.1; CAI41310.1; CAI41310.1; CAI41310.1; CAI41310.1; EAW47587.1; AAI36277.1; AAI43736.1; AAI46771.1

Protein Name

Mitogen-activated protein kinase kinase kinase 4

Protein Synonyms/Alias

MAP three kinase 1; MAPK/ERK kinase kinase 4; MEK kinase 4; MEKK 4

Gene Name

MAP3K4

Gene Synonyms/Alias

KIAA0213; MAPKKK4; MEKK4; MTK1

Created Date

July 27, 2013

Organism

Homo sapiens (Human)

NCBI Taxa ID

9606

Lysine Modification

Position	Peptide	Type	References
890	AAGKDCSKDSDDVLI	acetylation	[1]

Reference

[1] Regulation of cellular metabolism by protein lysine acetylation.
Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]

Functional Description

Component of a protein kinase signal transduction cascade. Activates the CSBP2, P38 and JNK MAPK pathways, but not the ERK pathway. Specifically phosphorylates and activates MAP2K4 and MAP2K6.

Sequence Annotation

DOMAIN 1343 1601 Protein kinase.
NP_BIND 1349 1357 ATP (By similarity).
ACT_SITE 1463 1463 Proton acceptor (By similarity).
BINDING 1372 1372 ATP (By similarity).
MOD_RES 456 456 Phosphoserine.
MOD_RES 457 457 Phosphoserine.
MOD_RES 458 458 Phosphothreonine.
MOD_RES 461 461 Phosphoserine.
MOD_RES 499 499 Phosphoserine.

Keyword

Alternative splicing; ATP-binding; Complete proteome; Cytoplasm; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase.

Sequence Source

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

Protein Length

1608 AA

Protein Sequence

MREAAAALVP PPAFAVTPAA AMEEPPPPPP PPPPPPEPET ESEPECCLAA RQEGTLGDSA 60
CKSPESDLED FSDETNTENL YGTSPPSTPR QMKRMSTKHQ RNNVGRPASR SNLKEKMNAP 120
NQPPHKDTGK TVENVEEYSY KQEKKIRAAL RTTERDRKKN VQCSFMLDSV GGSLPKKSIP 180
DVDLNKPYLS LGCSNAKLPV SVPMPIARPA RQTSRTDCPA DRLKFFETLR LLLKLTSVSK 240
KKDREQRGQE NTSGFWLNRS NELIWLELQA WHAGRTINDQ DFFLYTARQA IPDIINEILT 300
FKVDYGSFAF VRDRAGFNGT SVEGQCKATP GTKIVGYSTH HEHLQRQRVS FEQVKRIMEL 360
LEYIEALYPS LQALQKDYEK YAAKDFQDRV QALCLWLNIT KDLNQKLRIM GTVLGIKNLS 420
DIGWPVFEIP SPRPSKGNEP EYEGDDTEGE LKELESSTDE SEEEQISDPR VPEIRQPIDN 480
SFDIQSRDCI SKKLERLESE DDSLGWGAPD WSTEAGFSRH CLTSIYRPFV DKALKQMGLR 540
KLILRLHKLM DGSLQRARIA LVKNDRPVEF SEFPDPMWGS DYVQLSRTPP SSEEKCSAVS 600
WEELKAMDLP SFEPAFLVLC RVLLNVIHEC LKLRLEQRPA GEPSLLSIKQ LVRECKEVLK 660
GGLLMKQYYQ FMLQEVLEDL EKPDCNIDAF EEDLHKMLMV YFDYMRSWIQ MLQQLPQASH 720
SLKNLLEEEW NFTKEITHYI RGGEAQAGKL FCDIAGMLLK STGSFLEFGL QESCAEFWTS 780
ADDSSASDEI RRSVIEISRA LKELFHEARE RASKALGFAK MLRKDLEIAA EFRLSAPVRD 840
LLDVLKSKQY VKVQIPGLEN LQMFVPDTLA EEKSIILQLL NAAAGKDCSK DSDDVLIDAY 900
LLLTKHGDRA RDSEDSWGTW EAQPVKVVPQ VETVDTLRSM QVDNLLLVVM QSAHLTIQRK 960
AFQQSIEGLM TLCQEQTSSQ PVIAKALQQL KNDALELCNR ISNAIDRVDH MFTSEFDAEV 1020
DESESVTLQQ YYREAMIQGY NFGFEYHKEV VRLMSGEFRQ KIGDKYISFA RKWMNYVLTK 1080
CESGRGTRPR WATQGFDFLQ AIEPAFISAL PEDDFLSLQA LMNECIGHVI GKPHSPVTGL 1140
YLAIHRNSPR PMKVPRCHSD PPNPHLIIPT PEGFSTRSMP SDARSHGSPA AAAAAAAAAV 1200
AASRPSPSGG DSVLPKSISS AHDTRGSSVP ENDRLASIAA ELQFRSLSRH SSPTEERDEP 1260
AYPRGDSSGS TRRSWELRTL ISQSKDTASK LGPIEAIQKS VRLFEEKRYR EMRRKNIIGQ 1320
VCDTPKSYDN VMHVGLRKVT FKWQRGNKIG EGQYGKVYTC ISVDTGELMA MKEIRFQPND 1380
HKTIKETADE LKIFEGIKHP NLVRYFGVEL HREEMYIFME YCDEGTLEEV SRLGLQEHVI 1440
RLYSKQITIA INVLHEHGIV HRDIKGANIF LTSSGLIKLG DFGCSVKLKN NAQTMPGEVN 1500
STLGTAAYMA PEVITRAKGE GHGRAADIWS LGCVVIEMVT GKRPWHEYEH NFQIMYKVGM 1560
GHKPPIPERL SPEGKDFLSH CLESDPKMRW TASQLLDHSF VKVCTDEE 1608

Gene Ontology

GO:0005737; C:cytoplasm; ISS:UniProtKB.
GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO:0004709; F:MAP kinase kinase kinase activity; TAS:ProtInc.
GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
GO:0060718; P:chorionic trophoblast cell differentiation; IEA:Compara.
GO:0007254; P:JNK cascade; TAS:ProtInc.
GO:0019100; P:male germ-line sex determination; IEA:Compara.
GO:0001890; P:placenta development; IEA:Compara.
GO:0043507; P:positive regulation of JUN kinase activity; ISS:UniProtKB.
GO:0010468; P:regulation of gene expression; IEA:Compara.

Interpro

IPR011009; Kinase-like_dom.
IPR000719; Prot_kinase_cat_dom.
IPR017441; Protein_kinase_ATP_BS.
IPR002290; Ser/Thr_dual-sp_kinase_dom.
IPR008271; Ser/Thr_kinase_AS.

Pfam

PF00069; Pkinase

SMART

SM00220; S_TKc

PROSITE

PS00107; PROTEIN_KINASE_ATP
PS50011; PROTEIN_KINASE_DOM
PS00108; PROTEIN_KINASE_ST

PRINTS