CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007011
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glutamine-dependent NAD(+) synthetase 
Protein Synonyms/Alias
 NAD(+) synthase [glutamine-hydrolyzing] 
Gene Name
 QNS1 
Gene Synonyms/Alias
 YHR074W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
323DPTVCPTKVREPFYHubiquitination[1]
393NGNEQVIKDVRKITRubiquitination[1]
535CLRGYLTKYDCSSADubiquitination[1]
551NPIGGISKTDLKRFIubiquitination[1]
692RFPWASRKIDEVVEQubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
  
Sequence Annotation
 DOMAIN 5 296 CN hydrolase.
 NP_BIND 359 366 ATP (By similarity).
 REGION 329 714 Ligase.
 ACT_SITE 361 361 By similarity.  
Keyword
 ATP-binding; Complete proteome; Ligase; NAD; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 714 AA 
Protein Sequence
MSHLITLATC NLNQWALDFE GNRDRILQSI KIAKERGARL RVGPELEITG YGCLDHFLEN 60
DVCLHSWEMY AQIIKNKETH GLILDIGMPV LHKNVRYNCR LLSLDGEILF IRPKIWLAND 120
GNYREMRFFT PWMKPGVVED FILPPEIQKV TGQRLVPFGD AVINSLDTCI GTETCEELFT 180
PQSPHIAMSL DGVEIMTNSS GSHHELRKLN KRLDLILNAT KRCGGVYLYA NQRGCDGDRL 240
YYDGCALIAI NGTIVAQGSQ FSLDDVEVVT ATVDLEEVRS YRAAVMSRGL QASLAEIKFK 300
RIDIPVELAL MTSRFDPTVC PTKVREPFYH SPEEEIALGP ACWMWDYLRR CNGTGFFLPL 360
SGGIDSCATA MIVHSMCRLV TDAAQNGNEQ VIKDVRKITR SGDDWIPDSP QDLASKIFHS 420
CFMGTENSSK ETRNRAKDLS NAIGSYHVDL KMDSLVSSVV SLFEVATGKK PIYKIFGGSQ 480
IENLALQNIQ ARLRMVLSYL FAQLLPWVRG IPNSGGLLVL GSANVDECLR GYLTKYDCSS 540
ADINPIGGIS KTDLKRFIAY ASKQYNMPIL NDFLNATPTA ELEPMTKDYV QSDEIDMGMT 600
YEELGVFGYL RKVEKCGPYS MFLKLLHQWS PKLTPRQISE KVKRFFFFYA INRHKQTVLT 660
PSYHAEQYSP EDNRFDLRPF LINPRFPWAS RKIDEVVEQC EAHKGSTLDI MSID 714 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; IDA:SGD.
 GO:0003952; F:NAD+ synthase (glutamine-hydrolyzing) activity; IDA:SGD.
 GO:0009435; P:NAD biosynthetic process; IDA:SGD. 
Interpro
 IPR003010; C-N_Hydrolase.
 IPR014445; Gln-dep_NAD_synthase.
 IPR022310; NAD/GMP_synthase.
 IPR003694; NAD_synthase.
 IPR014729; Rossmann-like_a/b/a_fold. 
Pfam
 PF00795; CN_hydrolase
 PF02540; NAD_synthase 
SMART
  
PROSITE
 PS50263; CN_HYDROLASE 
PRINTS