CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023233
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rho GTPase-activating protein 26 
Protein Synonyms/Alias
 GTPase regulator associated with focal adhesion kinase; Oligophrenin-1-like protein; Rho-type GTPase-activating protein 26 
Gene Name
 ARHGAP26 
Gene Synonyms/Alias
 GRAF; KIAA0621; OPHN1L 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
51KSLISALKNLSSAKRubiquitination[1]
59NLSSAKRKFADSLNEubiquitination[1]
118VLITPLEKFRKEQIGubiquitination[1, 2]
254ESLMKKMKENPLEHKubiquitination[1]
310FDQKSGGKGGEDESVubiquitination[2]
454KTITSALKTYLRMLPubiquitination[1, 3, 4, 5]
475QFQRSFIKAAKLENQubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789
Functional Description
 GTPase-activating protein for RHOA and CDC42. 
Sequence Annotation
 DOMAIN 265 369 PH.
 DOMAIN 383 568 Rho-GAP.
 DOMAIN 756 814 SH3.  
Keyword
 3D-structure; Alternative splicing; Cell junction; Chromosomal rearrangement; Complete proteome; Cytoplasm; Cytoskeleton; Disease mutation; GTPase activation; Proto-oncogene; Reference proteome; SH3 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 814 AA 
Protein Sequence
MGLPALEFSD CCLDSPHFRE TLKSHEAELD KTNKFIKELI KDGKSLISAL KNLSSAKRKF 60
ADSLNEFKFQ CIGDAETDDE MCIARSLQEF ATVLRNLEDE RIRMIENASE VLITPLEKFR 120
KEQIGAAKEA KKKYDKETEK YCGILEKHLN LSSKKKESQL QEADSQVDLV RQHFYEVSLE 180
YVFKVQEVQE RKMFEFVEPL LAFLQGLFTF YHHGYELAKD FGDFKTQLTI SIQNTRNRFE 240
GTRSEVESLM KKMKENPLEH KTISPYTMEG YLYVQEKRHF GTSWVKHYCT YQRDSKQITM 300
VPFDQKSGGK GGEDESVILK SCTRRKTDSI EKRFCFDVEA VDRPGVITMQ ALSEEDRRLW 360
MEAMDGREPV YNSNKDSQSE GTAQLDSIGF SIIRKCIHAV ETRGINEQGL YRIVGVNSRV 420
QKLLSVLMDP KTASETETDI CAEWEIKTIT SALKTYLRML PGPLMMYQFQ RSFIKAAKLE 480
NQESRVSEIH SLVHRLPEKN RQMLQLLMNH LANVANNHKQ NLMTVANLGV VFGPTLLRPQ 540
EETVAAIMDI KFQNIVIEIL IENHEKIFNT VPDMPLTNAQ LHLSRKKSSD SKPPSCSERP 600
LTLFHTVQST EKQEQRNSII NSSLESVSSN PNSILNSSSS LQPNMNSSDP DLAVVKPTRP 660
NSLPPNPSPT SPLSPSWPMF SAPSSPMPTS STSSDSSPVR SVAGFVWFSV AAVVLSLARS 720
SLHAVFSLLV NFVPCHPNLH LLFDRPEEAV HEDSSTPFRK AKALYACKAE HDSELSFTAG 780
TVFDNVHPSQ EPGWLEGTLN GKTGLIPENY VEFL 814 
Gene Ontology
 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005925; C:focal adhesion; IEA:UniProtKB-SubCell.
 GO:0005543; F:phospholipid binding; IEA:InterPro.
 GO:0005100; F:Rho GTPase activator activity; NAS:UniProtKB.
 GO:0030036; P:actin cytoskeleton organization; NAS:UniProtKB.
 GO:0046847; P:filopodium assembly; IEA:InterPro.
 GO:0007399; P:nervous system development; NAS:UniProtKB.
 GO:0007264; P:small GTPase mediated signal transduction; TAS:Reactome. 
Interpro
 IPR013606; IRSp53/MIM_homology_IMD.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR008936; Rho_GTPase_activation_prot.
 IPR000198; RhoGAP_dom.
 IPR001452; SH3_domain. 
Pfam
 PF08397; IMD
 PF00620; RhoGAP
 PF00018; SH3_1 
SMART
 SM00233; PH
 SM00324; RhoGAP
 SM00326; SH3 
PROSITE
 PS50003; PH_DOMAIN
 PS50238; RHOGAP
 PS50002; SH3 
PRINTS