UniProt Accession

 ELF4_HUMAN; Q99607; D3DTG1; O60435 

Genbank Protein ID

 U32645; AF000670; AL136450; Z81363; Z81363; AL136450; CH471107; CH471107; BC017194 

Genbank Nucleotide ID

 AAB53693.1; AAC17452.1; CAI42882.1; CAI42882.1; CAI42371.1; CAI42371.1; EAX11813.1; EAX11814.1; AAH17194.1 

Protein Name

 ETS-related transcription factor Elf-4 

Protein Synonyms/Alias

 E74-like factor 4; Myeloid Elf-1-like factor 

Gene Name

 ELF4 

Gene Synonyms/Alias

 ELFR; MEF 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
240	QREKGIFKLVDSKAV	ubiquitination	[1, 2]
291	QRLVYQFKEMPKDLV	ubiquitination	[1, 2]
537	TTAAPRVKEGPLRSS	ubiquitination	[3]
657	FNPTSLIKMEPHDI*	sumoylation	[4]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] SUMO down-regulates the activity of Elf4/myeloid Elf-1-like factor.
 Suico MA, Nakamura H, Lu Z, Saitoh H, Shuto T, Nakao M, Kai H.
 Biochem Biophys Res Commun. 2006 Sep 29;348(3):880-8. [PMID: 16904644] 

Functional Description

 Transcriptional activator that binds to DNA sequences containing the consensus 5'-WGGA-3'. Transactivates promoters of the hematopoietic growth factor genes CSF2, IL3, IL8, and of the bovine lysozyme gene. Acts synergistically with RUNX1 to transactivate the IL3 promoter (By similarity). Also transactivates the PRF1 promoter in natural killer (NK) cells. Plays a role in the development and function of NK and NK T-cells and in innate immunity. Controls the proliferation and homing of CD8+ T-cells via the Kruppel-like factors KLF4 and KLF2 (By similarity). Controls cell senescence in a p53-dependent manner. Can also promote cellular transformation through inhibition of the p16 pathway. 

Sequence Annotation

 DNA_BIND 209 291 ETS.
 REGION 87 206 RUNX1-binding.
 MOD_RES 151 151 Phosphoserine.
 MOD_RES 648 648 Phosphoserine.  

Keyword

 Activator; Chromosomal rearrangement; Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Proto-oncogene; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 663 AA 

Protein Sequence

Gene Ontology

 GO:0016605; C:PML body; IDA:UniProtKB.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; NAS:UniProtKB.
 GO:0001787; P:natural killer cell proliferation; ISS:UniProtKB.
 GO:0001866; P:NK T cell proliferation; ISS:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR000418; Ets_dom.
 IPR022084; TF_Elf_N.
 IPR011991; WHTH_DNA-bd_dom. 

Pfam

 PF12310; Elf-1_N
 PF00178; Ets 

SMART

 SM00413; ETS 

PROSITE

 PS00345; ETS_DOMAIN_1
 PS00346; ETS_DOMAIN_2
 PS50061; ETS_DOMAIN_3 

PRINTS

 PR00454; ETSDOMAIN.