UniProt Accession

 TRF5_YEAST; P48561; D6W0P5 

Genbank Protein ID

 U47282; U23084; Z71575; BK006947 

Genbank Nucleotide ID

 AAC49397.1; AAC49099.1; CAA96217.1; DAA10261.1 

Protein Name

 Poly(A) RNA polymerase protein 1 

Protein Synonyms/Alias

 Topoisomerase 1-related protein TRF5 

Gene Name

 TRF5 

Gene Synonyms/Alias

 YNL299W; N0440 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
477	SILGNVIKYRGQKRD	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Catalytic subunit of the TRAMP5 complex which has a poly(A) RNA polymerase activity and is involved in a post- transcriptional quality control mechanism limiting inappropriate expression of genetic information. Polyadenylation is required for the degradative activity of the exosome on several of its nuclear RNA substrates like cryptic transcripts generated by RNA polymerase II and III, or hypomethylated pre-tRNAi-Met. Polyadenylates RNA processing and degradation intermediates of snRNAs, snoRNAs and mRNAs that accumulate in strains lacking a functional exosome. TRF5 is also required for proper nuclear division in mitosis and sister chromatid cohesion. Involved in the regulation of histone mRNA levels. May mediate mitotic chromosome condensation. 

Sequence Annotation

 DOMAIN 368 428 PAP-associated.
 METAL 233 233 Magnesium or manganese; catalytic (By
 METAL 235 235 Magnesium or manganese; catalytic (By
 MOD_RES 596 596 Phosphoserine.
 MOD_RES 602 602 Phosphoserine.  

Keyword

 Cell cycle; Cell division; Complete proteome; Magnesium; Manganese; Metal-binding; Mitosis; Nucleotidyltransferase; Nucleus; Phosphoprotein; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 642 AA 

Protein Sequence

Gene Ontology

 GO:0005730; C:nucleolus; IDA:SGD.
 GO:0031499; C:TRAMP complex; IDA:SGD.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004652; F:polynucleotide adenylyltransferase activity; IDA:SGD.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0071044; P:histone mRNA catabolic process; IGI:SGD.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0071039; P:nuclear polyadenylation-dependent CUT catabolic process; IGI:SGD.
 GO:0071042; P:nuclear polyadenylation-dependent mRNA catabolic process; IGI:SGD.
 GO:0071035; P:nuclear polyadenylation-dependent rRNA catabolic process; IMP:SGD.
 GO:0071036; P:nuclear polyadenylation-dependent snoRNA catabolic process; IGI:SGD.
 GO:0071037; P:nuclear polyadenylation-dependent snRNA catabolic process; IGI:SGD.
 GO:0071038; P:nuclear polyadenylation-dependent tRNA catabolic process; IGI:SGD.
 GO:0071051; P:polyadenylation-dependent snoRNA 3'-end processing; IGI:SGD.
 GO:0071050; P:snoRNA polyadenylation; IGI:SGD.
 GO:0034475; P:U4 snRNA 3'-end processing; IMP:SGD. 

Interpro

 IPR002934; Nucleotidyltransferase.
 IPR002058; PAP_assoc. 

Pfam

 PF01909; NTP_transf_2
 PF03828; PAP_assoc 

SMART

  

PROSITE

  

PRINTS