CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014504
UniProt Accession
Genbank Protein ID
 L23874 
Genbank Nucleotide ID
Protein Name
 Cytoplasmic aconitate hydratase 
Protein Synonyms/Alias
 Aconitase; Citrate hydro-lyase; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1 
Gene Name
 Aco1 
Gene Synonyms/Alias
 Ireb1; Irebp 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
621QKIETVNKSWNALAAubiquitination[1]
Reference
 [1] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding (By similarity). 
Sequence Annotation
 REGION 205 207 Substrate binding (By similarity).
 REGION 779 780 Substrate binding (By similarity).
 METAL 437 437 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 503 503 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 506 506 Iron-sulfur (4Fe-4S) (By similarity).
 BINDING 86 86 Substrate (By similarity).
 BINDING 536 536 Substrate (By similarity).
 BINDING 541 541 Substrate (By similarity).
 BINDING 699 699 Substrate (By similarity).  
Keyword
 4Fe-4S; Complete proteome; Cytoplasm; Direct protein sequencing; Iron; Iron-sulfur; Lyase; Metal-binding; Reference proteome; RNA-binding; Tricarboxylic acid cycle. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 889 AA 
Protein Sequence
MKNPFAHLAE PLDPAQPGKK FFNLNKLEDS RYGRLPFSIR VLLEAAVRNC DEFLVKKNDI 60
ENILNWSIMQ HKSIEVPFKP ARVILQDFTG VPAVVDFAAM RDAVKKLGGN PEKINPVCPA 120
DLVIDHSIQV HFNRRADSLQ KNQDLEFERN RERFEFLKWG SQAFCNMRII PPGSGIIHQV 180
NLEYLARVVF DQDGCYYPDS LVGTDSHTTM IDGLGVLGWG VGGIEAEAVM LGQPISMVLP 240
QVIGYKLMGK PHPLVTSTDI VLTITKHLRQ VGVVGKFVEF FGPGVAQLSI ADRATIANMC 300
PEYGATAAFF PVDDVSIAYL VQTGREEDKV KHIKRYLQAV GMFRDFSDSS QDPDFTQVVE 360
LDLKTVVPCC SGPKRPQDKV AVSEIEKDFE SCLGAKQGFK GFQVAPDHHN DHKTFIYNDS 420
EFTLAHGSVV IAAITSCTNT SNPSVMLGAG LLAKKAVEAG LNVKPYVKTS LSPGSGVVTY 480
YLRESGVMPY LSQLGFDVVG YGCMTCIGNS GPLPEPVVEA ITQGDLVAVG VLSGNRNFEG 540
RVHPNTRANY LASPPLVIAY AIAGTVRIDF EKEPLGVNAQ GQQVFLKDIW PTRDEIQEVE 600
RKYVIPGMFK EVYQKIETVN KSWNALAAPS EKLYAWNPKS TYIKSPPFFE SLTLDLQPPK 660
SIVDAYVLLN LGDSVTTDHI SPAGNIARNS PAARYLTNRG LTPRDFNSYG SRRGNDAIMA 720
RGTFANIRLL NKFLNKQAPQ TVHLPSGETL DVFDAAERYQ QAGLPLIVLA GKEYGSGSSR 780
DWAAKGPFLL GIKAVLAESY ERTHCSNLVG MGVIPLEYLP GETADSLGLT GRERYTIHIP 840
EHLKPRMKVQ IKLDTGKTFQ AVMRFDTDVE LTYFHNGGIL NYMIRKMAQ 889 
Gene Ontology
 GO:0005829; C:cytosol; IDA:RGD.
 GO:0005783; C:endoplasmic reticulum; IDA:RGD.
 GO:0005794; C:Golgi apparatus; IDA:MGI.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; ISS:UniProtKB.
 GO:0003994; F:aconitate hydratase activity; ISS:UniProtKB.
 GO:0052632; F:citrate hydro-lyase (cis-aconitate-forming) activity; IEA:EC.
 GO:0030350; F:iron-responsive element binding; ISS:UniProtKB.
 GO:0052633; F:isocitrate hydro-lyase (cis-aconitate-forming) activity; IEA:EC.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0048027; F:mRNA 5'-UTR binding; IDA:RGD.
 GO:0006879; P:cellular iron ion homeostasis; IDA:RGD.
 GO:0006101; P:citrate metabolic process; ISS:UniProtKB.
 GO:0006417; P:regulation of translation; TAS:RGD.
 GO:0010040; P:response to iron(II) ion; ISS:UniProtKB.
 GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW. 
Interpro
 IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
 IPR015937; Acoase/IPM_deHydtase.
 IPR001030; Acoase/IPM_deHydtase_lsu_aba.
 IPR015928; Aconitase/3IPM_dehydase_swvl.
 IPR006249; Aconitase/Fe_reg_prot_2.
 IPR015934; Aconitase/Fe_reg_prot_2/AcnD.
 IPR015932; Aconitase/IPMdHydase_lsu_aba_2.
 IPR018136; Aconitase_4Fe-4S_BS.
 IPR000573; AconitaseA/IPMdHydase_ssu_swvl. 
Pfam
 PF00330; Aconitase
 PF00694; Aconitase_C 
SMART
  
PROSITE
 PS00450; ACONITASE_1
 PS01244; ACONITASE_2 
PRINTS
 PR00415; ACONITASE.