CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006521
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nuclear pore complex protein Nup214 
Protein Synonyms/Alias
 214 kDa nucleoporin; Nucleoporin Nup214; Protein CAN 
Gene Name
 NUP214 
Gene Synonyms/Alias
 CAIN; CAN; KIAA0023 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
143KRPFAYHKLLKDAGGacetylation[1]
226YLPTLQEKKVIPCPPacetylation[2]
665PVPSMVQKSPRITPPubiquitination[3]
691LQPAVAEKQGHQWKDacetylation[4]
691LQPAVAEKQGHQWKDubiquitination[3]
930TTIESHTKSLPKVPAubiquitination[3]
953QLRNFLAKRKTPPVRubiquitination[3]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 May serve as a docking site in the receptor-mediated import of substrates across the nuclear pore complex. 
Sequence Annotation
 REPEAT 41 93 Blade 1.
 REPEAT 94 150 Blade 2.
 REPEAT 151 193 Blade 3.
 REPEAT 194 239 Blade 4.
 REPEAT 240 303 Blade 5.
 REPEAT 304 359 Blade 6.
 REPEAT 360 404 Blade 7.
 REGION 41 404 Seven-bladed beta propeller.
 REGION 481 2076 11 X 5 AA approximate repeats.
 REGION 740 768 Leucine-zipper 1.
 REGION 861 882 Leucine-zipper 2.
 REGION 1409 2084 18 X 4 AA approximate repeats.
 REGION 1427 2085 11 X 3 AA approximate repeats.
 MOD_RES 430 430 Phosphoserine.
 MOD_RES 433 433 Phosphoserine.
 MOD_RES 434 434 Phosphothreonine.
 MOD_RES 437 437 Phosphothreonine.
 MOD_RES 439 439 Phosphothreonine.
 MOD_RES 651 651 Phosphoserine.
 MOD_RES 657 657 Phosphoserine.
 MOD_RES 666 666 Phosphoserine.
 MOD_RES 670 670 Phosphothreonine.
 MOD_RES 678 678 Phosphoserine.
 MOD_RES 940 940 Phosphoserine.
 MOD_RES 970 970 Phosphoserine.
 MOD_RES 974 974 Phosphoserine.
 MOD_RES 989 989 Phosphoserine.
 MOD_RES 1023 1023 Phosphoserine.
 MOD_RES 1045 1045 Phosphoserine.
 MOD_RES 1081 1081 Phosphoserine.
 MOD_RES 1134 1134 Phosphothreonine.
 MOD_RES 1150 1150 Phosphothreonine.
 MOD_RES 1156 1156 Phosphothreonine.
 MOD_RES 1181 1181 Phosphoserine.
 MOD_RES 1312 1312 Phosphothreonine.
 MOD_RES 1963 1963 Phosphoserine.
 MOD_RES 1985 1985 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Chromosomal rearrangement; Coiled coil; Complete proteome; Glycoprotein; mRNA transport; Nuclear pore complex; Nucleus; Phosphoprotein; Polymorphism; Protein transport; Proto-oncogene; Reference proteome; Repeat; Translocation; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 2090 AA 
Protein Sequence
MGDEMDAMIP EREMKDFQFR ALKKVRIFDS PEELPKERSS LLAVSNKYGL VFAGGASGLQ 60
IFPTKNLLIQ NKPGDDPNKI VDKVQGLLVP MKFPIHHLAL SCDNLTLSAC MMSSEYGSII 120
AFFDVRTFSN EAKQQKRPFA YHKLLKDAGG MVIDMKWNPT VPSMVAVCLA DGSIAVLQVT 180
ETVKVCATLP STVAVTSVCW SPKGKQLAVG KQNGTVVQYL PTLQEKKVIP CPPFYESDHP 240
VRVLDVLWIG TYVFAIVYAA ADGTLETSPD VVMALLPKKE EKHPEIFVNF MEPCYGSCTE 300
RQHHYYLSYI EEWDLVLAAS AASTEVSILA RQSDQINWES WLLEDSSRAE LPVTDKSDDS 360
LPMGVVVDYT NQVEITISDE KTLPPAPVLM LLSTDGVLCP FYMINQNPGV KSLIKTPERL 420
SLEGERQPKS PGSTPTTPTS SQAPQKLDAS AAAAPASLPP SSPAAPIATF SLLPAGGAPT 480
VFSFGSSSLK SSATVTGEPP SYSSGSDSSK AAPGPGPSTF SFVPPSKASL APTPAASPVA 540
PSAASFSFGS SGFKPTLEST PVPSVSAPNI AMKPSFPPST SAVKVNLSEK FTAAATSTPV 600
SSSQSAPPMS PFSSASKPAA SGPLSHPTPL SAPPSSVPLK SSVLPSPSGR SAQGSSSPVP 660
SMVQKSPRIT PPAAKPGSPQ AKSLQPAVAE KQGHQWKDSD PVMAGIGEEI AHFQKELEEL 720
KARTSKACFQ VGTSEEMKML RTESDDLHTF LLEIKETTES LHGDISSLKT TLLEGFAGVE 780
EAREQNERNR DSGYLHLLYK RPLDPKSEAQ LQEIRRLHQY VKFAVQDVND VLDLEWDQHL 840
EQKKKQRHLL VPERETLFNT LANNREIINQ QRKRLNHLVD SLQQLRLYKQ TSLWSLSSAV 900
PSQSSIHSFD SDLESLCNAL LKTTIESHTK SLPKVPAKLS PMKQAQLRNF LAKRKTPPVR 960
STAPASLSRS AFLSQRYYED LDEVSSTSSV SQSLESEDAR TSCKDDEAVV QAPRHAPVVR 1020
TPSIQPSLLP HAAPFAKSHL VHGSSPGVMG TSVATSASKI IPQGADSTML ATKTVKHGAP 1080
SPSHPISAPQ AAAAAALRRQ MASQAPAVNT LTESTLKNVP QVVNVQELKN NPATPSTAMG 1140
SSVPYSTAKT PHPVLTPVAA NQAKQGSLIN SLKPSGPTPA SGQLSSGDKA SGTAKIETAV 1200
TSTPSASGQF SKPFSFSPSG TGFNFGIITP TPSSNFTAAQ GATPSTKESS QPDAFSSGGG 1260
SKPSYEAIPE SSPPSGITSA SNTTPGEPAA SSSRPVAPSG TALSTTSSKL ETPPSKLGEL 1320
LFPSSLAGET LGSFSGLRVG QADDSTKPTN KASSTSLTST QPTKTSGVPS GFNFTAPPVL 1380
GKHTEPPVTS SATTTSVAPP AATSTSSTAV FGSLPVTSAG SSGVISFGGT SLSAGKTSFS 1440
FGSQQTNSTV PPSAPPPTTA ATPLPTSFPT LSFGSLLSSA TTPSLPMSAG RSTEEATSSA 1500
LPEKPGDSEV SASAASLLEE QQSAQLPQAP PQTSDSVKKE PVLAQPAVSN SGTAASSTSL 1560
VALSAEATPA TTGVPDARTE AVPPASSFSV PGQTAVTAAA ISSAGPVAVE TSSTPIASST 1620
TSIVAPGPSA EAAAFGTVTS GSSVFAQPPA ASSSSAFNQL TNNTATAPSA TPVFGQVAAS 1680
TAPSLFGQQT GSTASTAAAT PQVSSSGFSS PAFGTTAPGV FGQTTFGQAS VFGQSASSAA 1740
SVFSFSQPGF SSVPAFGQPA SSTPTSTSGS VFGAASSTSS SSSFSFGQSS PNTGGGLFGQ 1800
SNAPAFGQSP GFGQGGSVFG GTSAATTTAA TSGFSFCQAS GFGSSNTGSV FGQAASTGGI 1860
VFGQQSSSSS GSVFGSGNTG RGGGFFSGLG GKPSQDAANK NPFSSASGGF GSTATSNTSN 1920
LFGNSGAKTF GGFASSSFGE QKPTGTFSSG GGSVASQGFG FSSPNKTGGF GAAPVFGSPP 1980
TFGGSPGFGG VPAFGSAPAF TSPLGSTGGK VFGEGTAAAS AGGFGFGSSS NTTSFGTLAS 2040
QNAPTFGSLS QQTSGFGTQS SGFSGFGSGT GGFSFGSNNS SVQGFGGWRS 2090 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005643; C:nuclear pore; TAS:ProtInc.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005487; F:nucleocytoplasmic transporter activity; IEA:Compara.
 GO:0005215; F:transporter activity; TAS:ProtInc.
 GO:0005975; P:carbohydrate metabolic process; TAS:Reactome.
 GO:0019221; P:cytokine-mediated signaling pathway; TAS:Reactome.
 GO:0010467; P:gene expression; TAS:Reactome.
 GO:0015758; P:glucose transport; TAS:Reactome.
 GO:0006406; P:mRNA export from nucleus; IEA:Compara.
 GO:0016071; P:mRNA metabolic process; TAS:Reactome.
 GO:0006611; P:protein export from nucleus; IMP:MGI.
 GO:0006606; P:protein import into nucleus; IEA:Compara.
 GO:0051726; P:regulation of cell cycle; IEA:Compara.
 GO:0010827; P:regulation of glucose transport; TAS:Reactome.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome.
 GO:0055085; P:transmembrane transport; TAS:Reactome.
 GO:0022415; P:viral reproductive process; TAS:Reactome. 
Interpro
 IPR026054; Nucleoporin.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat. 
Pfam
  
SMART
 SM00320; WD40 
PROSITE
  
PRINTS