CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008489
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rap1 GTPase-GDP dissociation stimulator 1 
Protein Synonyms/Alias
 Exchange factor smgGDS; SMG GDS protein; SMG P21 stimulatory GDP/GTP exchange protein 
Gene Name
 RAP1GDS1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
9DNLSDTLKKLKITAVubiquitination[1, 2]
10NLSDTLKKLKITAVDubiquitination[2]
67PQSSCKAKVANIIAEubiquitination[2]
77NIIAEVAKNEFMRIPubiquitination[2]
101LVQLLNSKDQEVLLQubiquitination[2]
230NIAEELVKLFKKQIEacetylation[3]
314QKLFEGGKGSVFQRVubiquitination[2]
421EMPPVQFKLLGTLRMacetylation[3]
421EMPPVQFKLLGTLRMubiquitination[2, 4, 5]
445EQLGKNVKLVERLVEubiquitination[2, 6, 7]
457LVEWCEAKDHAGVMGubiquitination[2, 7]
590KLRSHENKSVAQQASubiquitination[2, 6, 7]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 Stimulates GDP/GTP exchange reaction of a group of small GTP-binding proteins (G proteins) including Rap1a/Rap1b, RhoA, RhoB and KRas, by stimulating the dissociation of GDP from and the subsequent binding of GTP to each small G protein. 
Sequence Annotation
 REPEAT 77 118 ARM 1.
 REPEAT 169 211 ARM 2.
 REPEAT 346 390 ARM 3.
 REPEAT 391 431 ARM 4.
 REPEAT 479 519 ARM 5.
 MOD_RES 230 230 N6-acetyllysine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; GTPase activation; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 607 AA 
Protein Sequence
MDNLSDTLKK LKITAVDKTE DSLEGCLDCL LQALAQNNTE TSEKIQASGI LQLFASLLTP 60
QSSCKAKVAN IIAEVAKNEF MRIPCVDAGL ISPLVQLLNS KDQEVLLQTG RALGNICYDS 120
HEGRSAVDQA GGAQIVIDHL RSLCSITDPA NEKLLTVFCG MLMNYSNEND SLQAQLINMG 180
VIPTLVKLLG IHCQNAALTE MCLVAFGNLA ELESSKEQFA STNIAEELVK LFKKQIEHDK 240
REMIFEVLAP LAENDAIKLQ LVEAGLVECL LEIVQQKVDS DKEDDITELK TGSDLMVLLL 300
LGDESMQKLF EGGKGSVFQR VLSWIPSNNH QLQLAGALAI ANFARNDANC IHMVDNGIVE 360
KLMDLLDRHV EDGNVTVQHA ALSALRNLAI PVINKAKMLS AGVTEAVLKF LKSEMPPVQF 420
KLLGTLRMLI DAQAEAAEQL GKNVKLVERL VEWCEAKDHA GVMGESNRLL SALIRHSKSK 480
DVIKTIVQSG GIKHLVTMAT SEHVIMQNEA LVALALIAAL ELGTAEKDLE SAKLVQILHR 540
LLADERSAPE IKYNSMVLIC ALMGSECLHK EVQDLAFLDV VSKLRSHENK SVAQQASLTE 600
QRLTVES 607 
Gene Ontology
 GO:0005096; F:GTPase activator activity; NAS:UniProtKB. 
Interpro
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR000225; Armadillo. 
Pfam
 PF00514; Arm 
SMART
 SM00185; ARM 
PROSITE
 PS50176; ARM_REPEAT 
PRINTS