CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008305
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Max-interacting protein 1 
Protein Synonyms/Alias
 Max interactor 1; Class C basic helix-loop-helix protein 11; bHLHc11 
Gene Name
 MXI1 
Gene Synonyms/Alias
 BHLHC11 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
158RLCLERLKVLIPLGPubiquitination[1]
192KLEEAERKSQHQLENubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
 Transcriptional repressor. MXI1 binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MXI1 thus antagonizes MYC transcriptional activity by competing for MAX. 
Sequence Annotation
 DOMAIN 67 119 bHLH.  
Keyword
 Alternative splicing; Complete proteome; Disease mutation; DNA-binding; Nucleus; Proto-oncogene; Reference proteome; Repressor; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 228 AA 
Protein Sequence
MGKRGRPRKE ARCEGAGLAP AAPPAVPPAV AAPQPPALPE DPAGAKPRCP FSDIFNTSEN 60
SMEKHINTFL QNVQILLEAA SYLEQIEKEN KKCEHGYASS FPSMPSPRLQ HSKPPRRLSR 120
AQKHSSGSSN TSTANRSTHN ELEKNRRAHL RLCLERLKVL IPLGPDCTRH TTLGLLNKAK 180
AHIKKLEEAE RKSQHQLENL EREQRFLKWR LEQLQGPQEM ERIRMDSIGS TISSDRSDSE 240
REEIEVDVES TEFSHGEVDN ISTTSISDID DHSSLPSIGS DEGYSSASVK LSFTS 295 
Gene Ontology
 GO:0005634; C:nucleus; TAS:ProtInc.
 GO:0003677; F:DNA binding; TAS:ProtInc.
 GO:0003714; F:transcription corepressor activity; TAS:ProtInc.
 GO:0042994; P:cytoplasmic sequestering of transcription factor; TAS:ProtInc.
 GO:0008285; P:negative regulation of cell proliferation; TAS:ProtInc.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR011598; bHLH_dom. 
Pfam
 PF00010; HLH 
SMART
 SM00353; HLH 
PROSITE
 PS50888; BHLH 
PRINTS