UniProt Accession

 SMUG1_HUMAN; Q53HV7; A8K2K9; O95862; Q0D2M0; Q8NB71; Q9BWC8 

Genbank Protein ID

 AF125182; AK001235; AK091468; AK222473; AK290274; AF489699; CH471054; CH471054; BC000417; BC088352; BC105607 

Genbank Nucleotide ID

 AAD17301.1; BAA91571.1; BAC03670.1; BAD96193.1; BAF82963.1; AAL86910.1; EAW96752.1; EAW96756.1; AAH00417.1; AAH88352.1; AAI05608.1 

Protein Name

 Single-strand selective monofunctional uracil DNA glycosylase 

Protein Synonyms/Alias

  

Gene Name

 SMUG1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
185	TPAELPAKQREQLLG	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Responsible for recognizing base lesions in the genome and initiating base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA and has a preference for single-stranded DNA substrates. The activity is greater against mismatches (U/G) than against matches (U/A). Excised uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5- hydroxymethyluracil (hmU)] in ssDNA and dsDNA but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine) and other oxidized damage. The activity is damage specificity and salt concentration-dependent. The general order of the preference for ssDNA and dsDNA is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at the low salt concentration. At the high concentration dsDNA (G pair) > dsDNA (A pair) > ssDNA. 

Sequence Annotation

 REGION 173 187 DNA binding (By similarity).
 BINDING 84 84 Substrate; via amide nitrogen (By
 BINDING 98 98 Substrate; via amide nitrogen.
 BINDING 163 163 Substrate.
 BINDING 239 239 Substrate.  

Keyword

 Alternative splicing; Complete proteome; Direct protein sequencing; DNA damage; DNA repair; DNA-binding; Glycosidase; Hydrolase; Nucleus; Polymorphism; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 270 AA 

Protein Sequence

Gene Ontology

 GO:0005730; C:nucleolus; IDA:HGNC.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0017065; F:single-strand selective uracil DNA N-glycosylase activity; IDA:HGNC.
 GO:0045008; P:depyrimidination; TAS:Reactome. 

Interpro

 IPR005122; Uracil-DNA_glycosylase-like. 

Pfam

 PF03167; UDG 

SMART

  

PROSITE

  

PRINTS