CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021037
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cell surface glycoprotein MUC18 
Protein Synonyms/Alias
 Gicerin; Melanoma cell adhesion molecule; Melanoma-associated antigen MUC18; CD146 
Gene Name
 Mcam 
Gene Synonyms/Alias
 Muc18 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
607ITLPPTRKSEFVVEVacetylation[1]
607ITLPPTRKSEFVVEVubiquitination[2]
618VVEVKSDKLPEEMALubiquitination[2]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration (By similarity). 
Sequence Annotation
 DOMAIN 24 131 Ig-like V-type 1.
 DOMAIN 141 244 Ig-like V-type 2.
 DOMAIN 246 332 Ig-like C2-type 1.
 DOMAIN 337 426 Ig-like C2-type 2.
 DOMAIN 432 512 Ig-like C2-type 3.
 MOD_RES 616 616 Phosphoserine (By similarity).
 CARBOHYD 58 58 N-linked (GlcNAc...) (Potential).
 CARBOHYD 510 510 N-linked (GlcNAc...) (Potential).
 DISULFID 50 118 By similarity.
 DISULFID 163 225 By similarity.
 DISULFID 274 322 By similarity.
 DISULFID 367 409 By similarity.
 DISULFID 454 501 By similarity.  
Keyword
 Alternative splicing; Cell adhesion; Cell membrane; Complete proteome; Disulfide bond; Glycoprotein; Immunoglobulin domain; Membrane; Phosphoprotein; Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 648 AA 
Protein Sequence
MGLPRLVCAF LFAACCCCRS ATGVPGEEKQ PTPTPDPVEV EVGNTALLKC GPAHPSGNFS 60
QVEWFLIHKE RQIPIFRVHQ GKGQSEPGEY EHRLSLHGPG ATLALSQVTP HDDRMFLCKS 120
KQPRPQDHYV QLQVYKAPEE PTIQANVLGI HVDIQELKEV ATCVGRNGYP IPQVIWYKNG 180
RPLQEEENRV HIQSSQTVES SGLYTLKSVL SARLVKEDKD AQFYCELSYR LPSGNRMKES 240
KEVTVPVLYP AEKVWVEVEP VGLLKEGDHV KIRCLTDGNP QPHFTINKKN PSTEEMEEES 300
TDENGLLSLE PAQKHHSGVY QCQSLDLETT VMLSSDPLEL LVNYVSDVQV DPTAPEVQEG 360
DSLTLTCKAE SNQDLEFEWL RDKTGQLLGK GPILQLNNVK REAGGRYLCV ASVPSVPGLN 420
RTRRVSVGIF GSPWMAAKER KVWAQENAML NLSCEASGHP QPTISWNING SATEWNPDPQ 480
TVVSTLNVLV TPELLETGAE CTASNSLGSN TTVIILKLVT LTTLTPDSSQ TTGLSTPTVS 540
PHSRANSTST EKKLPQQESK GVVIVAVIVC TLVLAVLGAT LYYFYKKGKL PCGRSGKQEI 600
TLPPTRKSEF VVEVKSDKLP EEMALLQGSN GDKRAPGDQG EKYIDLRH 648 
Gene Ontology
 GO:0009897; C:external side of plasma membrane; IEA:Compara.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0043204; C:perikaryon; IEA:UniProtKB-SubCell.
 GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
 GO:0003094; P:glomerular filtration; IEA:Compara.
 GO:0061042; P:vascular wound healing; IEA:Compara. 
Interpro
 IPR013162; CD80_C2-set.
 IPR007110; Ig-like_dom.
 IPR013783; Ig-like_fold.
 IPR013098; Ig_I-set.
 IPR003599; Ig_sub.
 IPR003598; Ig_sub2.
 IPR013106; Ig_V-set.
 IPR013151; Immunoglobulin. 
Pfam
 PF08205; C2-set_2
 PF07679; I-set
 PF00047; ig
 PF07686; V-set 
SMART
 SM00409; IG
 SM00408; IGc2 
PROSITE
 PS50835; IG_LIKE 
PRINTS