CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000713
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Membrane-bound transcription factor site-2 protease 
Protein Synonyms/Alias
 Endopeptidase S2P; Sterol regulatory element-binding proteins intramembrane protease; SREBPs intramembrane protease 
Gene Name
 MBTPS2 
Gene Synonyms/Alias
 S2P 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
31LKSSVYFKHSYEDWLubiquitination[1]
363HTCLPARKAVEATQVubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Intramembrane proteolysis of sterol-regulatory element- binding proteins (SREBPs) within the first transmembrane segment thereby releasing the N-terminal segment with a portion of the transmembrane segment attached. Site-2 cleavage comes after site-1 cleavage which takes place in the lumenal loop. 
Sequence Annotation
 ACT_SITE 172 172
 METAL 171 171 Zinc; catalytic.
 METAL 175 175 Zinc; catalytic.
 CARBOHYD 337 337 N-linked (GlcNAc...).  
Keyword
 Cholesterol metabolism; Complete proteome; Cytoplasm; Disease mutation; Glycoprotein; Hydrolase; Ichthyosis; Lipid metabolism; Membrane; Metal-binding; Metalloprotease; Protease; Reference proteome; Steroid metabolism; Sterol metabolism; Transmembrane; Transmembrane helix; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 519 AA 
Protein Sequence
MIPVSLVVVV VGGWTVVYLT DLVLKSSVYF KHSYEDWLEN NGLSISPFHI RWQTAVFNRA 60
FYSWGRRKAR MLYQWFNFGM VFGVIAMFSS FFLLGKTLMQ TLAQMMADSP SSYSSSSSSS 120
SSSSSSSSSS SSSSSSLHNE QVLQVVVPGI NLPVNQLTYF FTAVLISGVV HEIGHGIAAI 180
REQVRFNGFG IFLFIIYPGA FVDLFTTHLQ LISPVQQLRI FCAGIWHNFV LALLGILALV 240
LLPVILLPFY YTGVGVLITE VAEDSPAIGP RGLFVGDLVT HLQDCPVTNV QDWNECLDTI 300
AYEPQIGYCI SASTLQQLSF PVRAYKRLDG STECCNNHSL TDVCFSYRNN FNKRLHTCLP 360
ARKAVEATQV CRTNKDCKKS SSSSFCIIPS LETHTRLIKV KHPPQIDMLY VGHPLHLHYT 420
VSITSFIPRF NFLSIDLPVV VETFVKYLIS LSGALAIVNA VPCFALDGQW ILNSFLDATL 480
TSVIGDNDVK DLIGFFILLG GSVLLAANVT LGLWMVTAR 519 
Gene Ontology
 GO:0000139; C:Golgi membrane; TAS:Reactome.
 GO:0016021; C:integral to membrane; TAS:ProtInc.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004222; F:metalloendopeptidase activity; TAS:ProtInc.
 GO:0006987; P:activation of signaling protein activity involved in unfolded protein response; TAS:Reactome.
 GO:0044267; P:cellular protein metabolic process; TAS:Reactome.
 GO:0008203; P:cholesterol metabolic process; TAS:ProtInc.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 
Interpro
 IPR008915; Peptidase_M50.
 IPR001193; SREBP_intramem_Prtase. 
Pfam
 PF02163; Peptidase_M50 
SMART
  
PROSITE
 PS00142; ZINC_PROTEASE 
PRINTS
 PR01000; SREBPS2PTASE.