UniProt Accession

 D3Z6I3_MOUSE; D3Z6I3 

Genbank Protein ID

 AC136513 

Genbank Nucleotide ID

  

Protein Name

 Lipoyl synthase, mitochondrial 

Protein Synonyms/Alias

 Lipoate synthase; Lipoic acid synthase 

Gene Name

 Lias 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
233	QPTKRHLKVEEYVTP	acetylation	[1, 2]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753] 

Functional Description

 Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives (By similarity). 

Sequence Annotation

 METAL 21 21 Iron-sulfur 1 (4Fe-4S) (By similarity).
 METAL 26 26 Iron-sulfur 1 (4Fe-4S) (By similarity).
 METAL 32 32 Iron-sulfur 1 (4Fe-4S) (By similarity).
 METAL 52 52 Iron-sulfur 2 (4Fe-4S-S-AdoMet) (By
 METAL 56 56 Iron-sulfur 2 (4Fe-4S-S-AdoMet) (By
 METAL 59 59 Iron-sulfur 2 (4Fe-4S-S-AdoMet) (By  

Keyword

 4Fe-4S; Complete proteome; Iron; Iron-sulfur; Metal-binding; Mitochondrion; Reference proteome; S-adenosyl-L-methionine; Transferase; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 289 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:HAMAP.
 GO:0016992; F:lipoate synthase activity; IGI:MGI.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0006954; P:inflammatory response; IMP:MGI.
 GO:0001843; P:neural tube closure; IMP:MGI.
 GO:0009249; P:protein lipoylation; IEA:HAMAP.
 GO:0032496; P:response to lipopolysaccharide; IMP:MGI.
 GO:0006979; P:response to oxidative stress; IMP:MGI. 

Interpro

 IPR013785; Aldolase_TIM.
 IPR006638; Elp3/MiaB/NifB.
 IPR003698; Lipoyl_synth.
 IPR007197; rSAM. 

Pfam

 PF04055; Radical_SAM 

SMART

 SM00729; Elp3 

PROSITE

  

PRINTS