CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000216
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Homogentisate 1,2-dioxygenase 
Protein Synonyms/Alias
 Homogentisate oxygenase; Homogentisic acid oxidase; Homogentisicase 
Gene Name
 Hgd 
Gene Synonyms/Alias
 Aku; Hgo 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
5***MAELKYISGFGNacetylation[1]
71ILPSVSHKPFESIDQubiquitination[2]
98DPNQLRWKPFEIPKAubiquitination[2]
104WKPFEIPKASEKKVDacetylation[3]
104WKPFEIPKASEKKVDubiquitination[2]
109IPKASEKKVDFVSGLubiquitination[2]
160DFLIVPQKGKLLIYTubiquitination[2]
162LIVPQKGKLLIYTEFubiquitination[2]
248GGYTVINKFQGKLFAubiquitination[2]
252VINKFQGKLFACKQDubiquitination[2]
327PRWGVADKTFRPPYYubiquitination[2]
353IKGHYEAKQGGFLPGubiquitination[2]
380PDADCFEKASKAKLEubiquitination[2]
414AVTKWGLKTCSCLDEacetylation[4]
414AVTKWGLKTCSCLDEsuccinylation[4]
414AVTKWGLKTCSCLDEubiquitination[2]
425CLDENYYKCWEPLRSubiquitination[2]
Reference
 [1] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 
Functional Description
  
Sequence Annotation
 METAL 335 335 Iron (By similarity).
 METAL 341 341 Iron (By similarity).
 METAL 371 371 Iron (By similarity).
 MOD_RES 98 98 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Complete proteome; Dioxygenase; Direct protein sequencing; Iron; Metal-binding; Oxidoreductase; Phenylalanine catabolism; Reference proteome; Tyrosine catabolism. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 445 AA 
Protein Sequence
MAELKYISGF GNECASEDPR CPGSLPKGQN NPQVCPYNLY AEQLSGSAFT CPRNTNKRSW 60
LYRILPSVSH KPFESIDQGH VTHNWDEVGP DPNQLRWKPF EIPKASEKKV DFVSGLYTLC 120
GAGDIKSNNG LAVHIFLCNS SMENRCFYNS DGDFLIVPQK GKLLIYTEFG KMSLQPNEIC 180
VIQRGMRFSV DVFEETRGYI LEVYGVHFEL PDLGPIGANG LANPRDFLIP VAWYEDRRVP 240
GGYTVINKFQ GKLFACKQDV SPFNVVAWHG NYTPYKYNLE NFMVINAVAF DHADPSIFTV 300
LTAKSLRPGV AIADFVIFPP RWGVADKTFR PPYYHRNCMS EFMGLIKGHY EAKQGGFLPG 360
GGSLHSAMTP HGPDADCFEK ASKAKLEPER IADGTMAFMF ESSLSLAVTK WGLKTCSCLD 420
ENYYKCWEPL RSHFTPNSRS PTEPK 445 
Gene Ontology
 GO:0004411; F:homogentisate 1,2-dioxygenase activity; IEA:EC.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0006520; P:cellular amino acid metabolic process; IMP:MGI.
 GO:0006559; P:L-phenylalanine catabolic process; IEA:UniProtKB-UniPathway.
 GO:0006572; P:tyrosine catabolic process; IEA:UniProtKB-KW. 
Interpro
 IPR005708; Homogentis_dOase.
 IPR014710; RmlC-like_jellyroll.
 IPR011051; RmlC_Cupin. 
Pfam
 PF04209; HgmA 
SMART
  
PROSITE
  
PRINTS