UniProt Accession

 PODXL_HUMAN; O00592; A6NHX8; Q52LZ7; Q53ER6 

Genbank Protein ID

 U97519; AK223573; AC008264; CH236950; CH471070; BC093730; BC112035; BP234810 

Genbank Nucleotide ID

 AAB61574.1; BAD97293.1; EAL24080.1; EAW83786.1; AAH93730.1; AAI12036.1 

Protein Name

 Podocalyxin 

Protein Synonyms/Alias

 GCTM-2 antigen; Gp200; Podocalyxin-like protein 1; PC; PCLP-1 

Gene Name

 PODXL 

Gene Synonyms/Alias

 PCLP; PCLP1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
525	TSSEMQEKKVVSLNG	ubiquitination	[1]
526	SSEMQEKKVVSLNGE	ubiquitination	[1, 2]
547	VPLDNLTKDDLDEEE	ubiquitination	[1, 3]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 

Functional Description

 Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell- cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR- dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. 

Sequence Annotation

 MOD_RES 529 529 Phosphoserine.
 MOD_RES 537 537 Phosphoserine.
 MOD_RES 556 556 Phosphothreonine.
 CARBOHYD 33 33 N-linked (GlcNAc...) (Potential).
 CARBOHYD 43 43 N-linked (GlcNAc...) (Potential).
 CARBOHYD 104 104 N-linked (GlcNAc...) (Potential).
 CARBOHYD 144 144 N-linked (GlcNAc...) (Potential).
 CARBOHYD 360 360 N-linked (GlcNAc...) (Potential).  

Keyword

 Alternative splicing; Cell adhesion; Cell membrane; Cell projection; Complete proteome; Direct protein sequencing; Glycoprotein; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Signal; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 558 AA 

Protein Sequence

Gene Ontology

 GO:0016324; C:apical plasma membrane; ISS:UniProtKB.
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0030175; C:filopodium; IDA:UniProtKB.
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0030027; C:lamellipodium; IDA:UniProtKB.
 GO:0045121; C:membrane raft; IEA:UniProtKB-SubCell.
 GO:0031528; C:microvillus membrane; ISS:UniProtKB.
 GO:0001726; C:ruffle; IDA:UniProtKB.
 GO:0036057; C:slit diaphragm; ISS:UniProtKB.
 GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
 GO:0016477; P:cell migration; ISS:UniProtKB.
 GO:0072175; P:epithelial tube formation; ISS:UniProtKB.
 GO:0072015; P:glomerular visceral epithelial cell development; ISS:UniProtKB.
 GO:0050900; P:leukocyte migration; IEA:Compara.
 GO:0022408; P:negative regulation of cell-cell adhesion; ISS:UniProtKB.
 GO:0030335; P:positive regulation of cell migration; IDA:UniProtKB.
 GO:0033634; P:positive regulation of cell-cell adhesion mediated by integrin; IDA:UniProtKB.
 GO:0032534; P:regulation of microvillus assembly; ISS:UniProtKB. 

Interpro

 IPR013836; CD34/Podocalyxin.
 IPR017403; Podocalyxin-like_p1. 

Pfam

 PF06365; CD34_antigen 

SMART

  

PROSITE

  

PRINTS