CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006714
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ribonucleoside-diphosphate reductase 2 subunit beta 
Protein Synonyms/Alias
 R2F protein; Ribonucleotide reductase 2 
Gene Name
 nrdF 
Gene Synonyms/Alias
 ygaD; b2676; JW2651 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
18WNKISDDKDLEVWNRacetylation[1]
177MYFSSRGKLTNTADLacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. R2F contains the tyrosyl radical required for catalysis. 
Sequence Annotation
 ACT_SITE 105 105 By similarity.
 METAL 67 67 Iron 1 (By similarity).
 METAL 98 98 Iron 1 (By similarity).
 METAL 98 98 Iron 2 (By similarity).
 METAL 101 101 Iron 1 (By similarity).
 METAL 158 158 Iron 2 (By similarity).
 METAL 192 192 Iron 2.
 METAL 195 195 Iron 2.  
Keyword
 3D-structure; Complete proteome; DNA replication; Iron; Metal-binding; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 319 AA 
Protein Sequence
MKLSRISAIN WNKISDDKDL EVWNRLTSNF WLPEKVPLSN DIPAWQTLTV VEQQLTMRVF 60
TGLTLLDTLQ NVIGAPSLMP DALTPHEEAV LSNISFMEAV HARSYSSIFS TLCQTKDVDA 120
AYAWSEENAP LQRKAQIIQQ HYRGDDPLKK KIASVFLESF LFYSGFWLPM YFSSRGKLTN 180
TADLIRLIIR DEAVHGYYIG YKYQKNMEKI SLGQREELKS FAFDLLLELY DNELQYTDEL 240
YAETPWADDV KAFLCYNANK ALMNLGYEPL FPAEMAEVNP AILAALSPNA DENHDFFSGS 300
GSSYVMGKAV ETEDEDWNF 319 
Gene Ontology
 GO:0005971; C:ribonucleoside-diphosphate reductase complex; IGI:EcoliWiki.
 GO:0005506; F:iron ion binding; ISA:EcoliWiki.
 GO:0030145; F:manganese ion binding; IDA:EcoCyc.
 GO:0004748; F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor; IEA:EC.
 GO:0009186; P:deoxyribonucleoside diphosphate metabolic process; IEA:InterPro.
 GO:0009263; P:deoxyribonucleotide biosynthetic process; IGI:EcoliWiki.
 GO:0006260; P:DNA replication; IEA:UniProtKB-UniPathway. 
Interpro
 IPR009078; Ferritin-like_SF.
 IPR012348; RNR-rel.
 IPR026494; RNR_1b_NrdF.
 IPR026023; RNR_bsu_prok.
 IPR000358; RNR_small. 
Pfam
 PF00268; Ribonuc_red_sm 
SMART
  
PROSITE
 PS00368; RIBORED_SMALL 
PRINTS