UniProt Accession

 RATA_ECOLI; P0AGL5; P52121 

Genbank Protein ID

 D12501; U36840; U00096; AP009048 

Genbank Nucleotide ID

 AAA79789.1; AAC75668.1; BAA16504.1 

Protein Name

 Ribosome association toxin RatA 

Protein Synonyms/Alias

 Toxin RatA 

Gene Name

 ratA 

Gene Synonyms/Alias

 pasT; yfjG; b2619; JW2600 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
68	TAAVDVSKAGISKTF	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Toxic component of a toxin-antitoxin (TA) module. Binds to 50S ribosomal subunits, preventing them from associating with 30S subunits to form 70S ribosomes and reducing polysomes. It does not cause ribosomes to dissociate however. The antibiotic paromomycin blocks the anti-association activity of RatA. Overexpression results in inhibition of growth in liquid cultures, and in a decrease in protein translation. The other gene of this operon, ratB, is not the cognate antitoxin in this strain; in CFT073 however it does fulfill this function. 

Sequence Annotation

  

Keyword

 Complete proteome; Reference proteome; Stress response; Toxin. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 158 AA 

Protein Sequence

Gene Ontology

 GO:0043023; F:ribosomal large subunit binding; IDA:EcoCyc.
 GO:0045947; P:negative regulation of translational initiation; IPI:EcoCyc.
 GO:0006950; P:response to stress; IEA:UniProtKB-KW. 

Interpro

 IPR005031; Polyket_cyc.
 IPR023393; START-like_dom. 

Pfam

 PF03364; Polyketide_cyc 

SMART

  

PROSITE

  

PRINTS