CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-027752
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Hepatocellular carcinoma-associated antigen 90 
Protein Synonyms/Alias
 TPX2, microtubule-associated, homolog (Xenopus laevis), isoform CRA_a; Targeting protein for Xklp2 
Gene Name
 HCA90 
Gene Synonyms/Alias
 TPX2; hCG_38821 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
44EKANLENKLLGKNGTubiquitination[1, 2, 3]
48LENKLLGKNGTGGLFubiquitination[1, 3, 4]
58TGGLFQGKTPLRKANacetylation[4]
58TGGLFQGKTPLRKANubiquitination[2]
63QGKTPLRKANLQQAIubiquitination[2]
75QAIVTPLKPVDNTYYacetylation[5, 6]
75QAIVTPLKPVDNTYYubiquitination[1, 2, 3]
128SLRLSAQKDLEQKEKacetylation[4]
305TKGCTIVKPFNLSQGacetylation[4, 6, 7]
305TKGCTIVKPFNLSQGubiquitination[8]
411QTPVLQTKHRARAVTacetylation[4]
512DVVGVPEKKVLPITVacetylation[4]
560PHYGVPFKPQIPEARacetylation[4, 7]
560PHYGVPFKPQIPEARubiquitination[1, 3]
603KGEVPKFKALPLPHFubiquitination[1, 3]
618DTINLPEKKVKNVTQacetylation[4]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Spermidine and resveratrol induce autophagy by distinct pathways converging on the acetylproteome.
 Morselli E, Mariño G, Bennetzen MV, Eisenberg T, Megalou E, Schroeder S, Cabrera S, Bénit P, Rustin P, Criollo A, Kepp O, Galluzzi L, Shen S, Malik SA, Maiuri MC, Horio Y, López-Otín C, Andersen JS, Tavernarakis N, Madeo F, Kroemer G.
 J Cell Biol. 2011 Feb 21;192(4):615-29. [PMID: 21339330]
 [6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [7] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [8] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 783 AA 
Protein Sequence
MSQVKSSYSY DAPSDFINFS SLDDEGDTQN IDSWFEEKAN LENKLLGKNG TGGLFQGKTP 60
LRKANLQQAI VTPLKPVDNT YYKEAEKENL VEQSIPSNAC SSLEVEAAIS RKTPAQPQRR 120
SLRLSAQKDL EQKEKHHVKM KAKRCATPVI IDEILPSKKM KVSNNKKKPE EEGSAHQDTA 180
EKNASSPEKA KGRHTVPCMP PAKQKFLKST EEQELEKSMK MQQEVVEMRK KNEEFKKLAL 240
AGIGQPVKKS VSQVTKSVDF HFRTDERIKQ HPKNQEEYKE VNFTSELRKH PSSPARVTKG 300
CTIVKPFNLS QGKKRTFDET VSTYVPLAQQ VEDFHKRTPN RYHLRSKKDD IKTGSCSVTQ 360
AGVQWRDHGS LQCPTPGLKQ SSCLSLPNLL PSKSSVTKIC RDPQTPVLQT KHRARAVTCK 420
STAELEAEEL EKLQQYKFKA RELDPRILEG GPILPKKPPV KPPTEPIGFD LEIEKRIQER 480
ESKKKTEDEH FEFHSRPCPT KILEDVVGVP EKKVLPITVP KSPAFALKNR IRMPTKEDEE 540
EDEPVVIKAQ PVPHYGVPFK PQIPEARTVE ICPFSFDSRD KERQLQKEKK IKELQKGEVP 600
KFKALPLPHF DTINLPEKKV KNVTQIEPFC LETDRRGALK AQTWKHQLEE ELRQQKEAAC 660
FKARPNTVIS QEPFVPKKEK KSVAEGLSGS LVQEPFQLAT EKRAKERQEL EKRMAEVEAQ 720
KAQQLEEARL QEEEQKKEEL ARLRRELVHK ANPIRKYQGL EIKSSDQPLT VPVSPKFSTR 780
FHC 783 
Gene Ontology
 GO:0005874; C:microtubule; IEA:InterPro.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005819; C:spindle; IEA:InterPro.
 GO:0007067; P:mitosis; IEA:InterPro. 
Interpro
 IPR015128; Aurora-A-bd.
 IPR027330; TPX2_central_dom.
 IPR027329; TPX2_dom_C.
 IPR009675; TPX2_fam. 
Pfam
 PF09041; Aurora-A_bind
 PF06886; TPX2
 PF12214; TPX2_importin 
SMART
  
PROSITE
  
PRINTS