CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-014903
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tubulin polyglutamylase TTLL5 
Protein Synonyms/Alias
 SRC1 and TIF2-associated modulatory protein; Tubulin--tyrosine ligase-like protein 5 
Gene Name
 TTLL5 
Gene Synonyms/Alias
 KIAA0998; STAMP 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
118RTLSEAQKVNHFPRSubiquitination[1]
180DRGPWIVKPVASSRGubiquitination[1]
258VRYDQGAKNIRNQFMubiquitination[1]
440SASDAEMKNLVGSARubiquitination[1]
453AREKGPGKLGGSVLGubiquitination[1]
812TFYTQKNKSASVFLGubiquitination[1]
826GTHSKISKNNNNYSDubiquitination[1]
837NYSDSGAKGDHPETIubiquitination[1]
875RFTTSAEKEAKLVYSubiquitination[1]
878TSAEKEAKLVYSNSSubiquitination[1]
1002AGSCYLNKHHSGIAKubiquitination[1]
1012SGIAKTQKEGEDASLubiquitination[1]
1022EDASLYSKRYNQSMVubiquitination[1]
1159LQQLEQQKLQSRQLLubiquitination[1]
1207SATASGQKPTTLPQKubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Polyglutamylase which preferentially modifies alpha- tubulin. Involved in the side-chain initiation step of the polyglutamylation reaction rather than in the elongation step (By similarity). Required for CCSAP localization to both spindle and cilia microtubules. Increases the effects of NCOA2 in glucocorticoid receptor-mediated repression and induction and in androgen receptor-mediated induction. 
Sequence Annotation
 DOMAIN 62 407 TTL.  
Keyword
 Alternative splicing; Cell projection; Cilium; Complete proteome; Cytoplasm; Cytoskeleton; Ligase; Nucleus; Polymorphism; Reference proteome; Transcription. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1281 AA 
Protein Sequence
MPIVMARDLE ETASSSEDEE VISQEDHPCI MWTGGCRRIP VLVFHADAIL TKDNNIRVIG 60
ERYHLSYKIV RTDSRLVRSI LTAHGFHEVH PSSTDYNLMW TGSHLKPFLL RTLSEAQKVN 120
HFPRSYELTR KDRLYKNIIR MQHTHGFKAF HILPQTFLLP AEYAEFCNSY SKDRGPWIVK 180
PVASSRGRGV YLINNPNQIS LEENILVSRY INNPLLIDDF KFDVRLYVLV TSYDPLVIYL 240
YEEGLARFAT VRYDQGAKNI RNQFMHLTNY SVNKKSGDYV SCDDPEVEDY GNKWSMSAML 300
RYLKQEGRDT TALMAHVEDL IIKTIISAEL AIATACKTFV PHRSSCFELY GFDVLIDSTL 360
KPWLLEVNLS PSLACDAPLD LKIKASMISD MFTVVGFVCQ DPAQRASTRP IYPTFESSRR 420
NPFQKPQRCR PLSASDAEMK NLVGSAREKG PGKLGGSVLG LSMEEIKVLR RVKEENDRRG 480
GFIRIFPTSE TWEIYGSYLE HKTSMNYMLA TRLFQDRMTA DGAPELKIES LNSKAKLHAA 540
LYERKLLSLE VRKRRRRSSR LRAMRPKYPV ITQPAEMNVK TETESEEEEE VALDNEDEEQ 600
EASQEESAGF LRENQAKYTP SLTALVENTP KENSMKVREW NNKGGHCCKL ETQELEPKFN 660
LMQILQDNGN LSKMQARIAF SAYLQHVQIR LMKDSGGQTF SASWAAKEDE QMELVVRFLK 720
RASNNLQHSL RMVLPSRRLA LLERRRILAH QLGDFIIVYN KETEQMAEKK SKKKVEEEEE 780
DGVNMENFQE FIRQASEAEL EEVLTFYTQK NKSASVFLGT HSKISKNNNN YSDSGAKGDH 840
PETIMEEVKI KPPKQQQTTE IHSDKLSRFT TSAEKEAKLV YSNSSSGPTA TLQKIPNTHL 900
SSVTTSDLSP GPCHHSSLSQ IPSAIPSMPH QPTILLNTVS ASASPCLHPG AQNIPSPTGL 960
PRCRSGSHTI GPFSSFQSAA HIYSQKLSRP SSAKAGSCYL NKHHSGIAKT QKEGEDASLY 1020
SKRYNQSMVT AELQRLAEKQ AARQYSPSSH INLLTQQVTN LNLATGIINR SSASAPPTLR 1080
PIISPSGPTW STQSDPQAPE NHSSSPGSRS LQTGGFAWEG EVENNVYSQA TGVVPQHKYH 1140
PTAGSYQLQF ALQQLEQQKL QSRQLLDQSR ARHQAIFGSQ TLPNSNLWTM NNGAGCRISS 1200
ATASGQKPTT LPQKVVPPPS SCASLVPKPP PNHEQVLRRA TSQKASKGSS AEGQLNGLQS 1260
SLNPAAFVPI TSSTDPAHTK I 1281 
Gene Ontology
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005929; C:cilium; IEA:UniProtKB-SubCell.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005932; C:microtubule basal body; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0004835; F:tubulin-tyrosine ligase activity; IEA:InterPro.
 GO:0006464; P:cellular protein modification process; IEA:InterPro.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR004344; Tub_tyr_ligase. 
Pfam
 PF03133; TTL 
SMART
  
PROSITE
 PS51221; TTL 
PRINTS