UniProt Accession

 PARL_HUMAN; Q9H300; Q96CQ4; Q9BTJ6; Q9P1E3 

Genbank Protein ID

 AF197937; BC003653; BC014058; AF116692 

Genbank Nucleotide ID

 AAG28519.1; AAH03653.1; AAH14058.1; AAF71112.1 

Protein Name

 Presenilins-associated rhomboid-like protein, mitochondrial 

Protein Synonyms/Alias

 Mitochondrial intramembrane cleaving protease PARL; P-beta; Pbeta 

Gene Name

 PARL 

Gene Synonyms/Alias

 PSARL; PRO2207 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
75	GTSGEAYKRSALIPP	ubiquitination	[1, 2, 3]
136	QSYFDGIKADWLDSI	ubiquitination	[1, 3]
362	KNREPLVKIWHEIRT	ubiquitination	[1, 3]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Required for the control of apoptosis during postnatal growth. Essential for proteolytic processing of an antiapoptotic form of OPA1 which prevents the release of mitochondrial cytochrome c in response to intrinsic apoptoptic signals (By similarity). Promotes changes in mitochondria morphology regulated by phosphorylation of P-beta domain. 

Sequence Annotation

 ACT_SITE 277 277 Nucleophile (By similarity).
 ACT_SITE 335 335 By similarity.
 MOD_RES 65 65 Phosphoserine.
 MOD_RES 69 69 Phosphothreonine.
 MOD_RES 70 70 Phosphoserine.  

Keyword

 Alternative splicing; Complete proteome; Direct protein sequencing; Hydrolase; Membrane; Mitochondrion; Mitochondrion inner membrane; Nucleus; Phosphoprotein; Polymorphism; Protease; Reference proteome; Serine protease; Transit peptide; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 379 AA 

Protein Sequence

Gene Ontology

 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
 GO:2001243; P:negative regulation of intrinsic apoptotic signaling pathway; IEA:Compara.
 GO:0090201; P:negative regulation of release of cytochrome c from mitochondria; IEA:Compara.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 

Interpro

 IPR002610; Peptidase_S54_rhomboid.
 IPR022764; Peptidase_S54_rhomboid_dom. 

Pfam

 PF01694; Rhomboid 

SMART

  

PROSITE

  

PRINTS