CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024107
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Alpha-actinin-1 
Protein Synonyms/Alias
 Alpha-actinin cytoskeletal isoform; F-actin cross-linking protein; Non-muscle alpha-actinin-1 
Gene Name
 Actn1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
89RGKMRVHKISNVNKAacetylation[1]
195PELIDYGKLRKDDPLacetylation[1]
195PELIDYGKLRKDDPLubiquitination[2]
402LAEKFRQKASIHEAWubiquitination[2]
420KEAMLRQKDYETATLacetylation[1]
431TATLSEIKALLKKHEacetylation[1]
563ATLPDADKERLAILGacetylation[1]
676SHLRQYEKSIVNYKPacetylation[1]
682EKSIVNYKPKIDQLEacetylation[1]
741QILTRDAKGISQEQMubiquitination[2]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (By similarity). 
Sequence Annotation
 DOMAIN 1 247 Actin-binding.
 DOMAIN 31 135 CH 1.
 DOMAIN 144 247 CH 2.
 REPEAT 274 384 Spectrin 1.
 REPEAT 394 499 Spectrin 2.
 REPEAT 509 620 Spectrin 3.
 REPEAT 630 733 Spectrin 4.
 DOMAIN 746 781 EF-hand 1.
 DOMAIN 787 822 EF-hand 2.
 REGION 274 733 Interaction with DDN (By similarity).
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 6 6 Phosphoserine (By similarity).
 MOD_RES 12 12 Phosphotyrosine; by FAK1 (By similarity).
 MOD_RES 95 95 N6-acetyllysine (By similarity).
 MOD_RES 195 195 N6-acetyllysine (By similarity).
 MOD_RES 676 676 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Actin-binding; Calcium; Cell membrane; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; Membrane; Metal-binding; Phosphoprotein; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 892 AA 
Protein Sequence
MDHYDSQQTN DYMQPEEDWD RDLLLDPAWE KQQRKTFTAW CNSHLRKAGT QIENIEEDFR 60
DGLKLMLLLE VISGERLAKP ERGKMRVHKI SNVNKALDFI ASKGVKLVSI GAEEIVDGNV 120
KMTLGMIWTI ILRFAIQDIS VEETSAKEGL LLWCQRKTAP YKNVNIQNFH ISWKDGLGFC 180
ALIHRHRPEL IDYGKLRKDD PLTNLNTAFD VAERYLDIPK MLDAEDIVGT ARPDEKAIMT 240
YVSSFYHAFS GAQKAETAAN RICKVLAVNQ ENEQLMEDYE KLASDLLEWI RRTIPWLENR 300
VPENTMQAMQ QKLEDFRDYR RLHKPPKVQE KCQLEINFNT LQTKLRLSNR PAFMPSEGRM 360
VSDINNAWGC LEQAEKGYEE WLLNEIRRLE RLDHLAEKFR QKASIHEAWT DGKEAMLRQK 420
DYETATLSEI KALLKKHEAF ESDLAAHQDR VEQIAAIAQE LNELDYYDSP SVNARCQKIC 480
DQWDNLGALT QKRREALERT EKLLETIDQL YLEYAKRAAP FNNWMEGAME DLQDTFIVHT 540
IEEIQGLTTA HEQFKATLPD ADKERLAILG IHNEVSKIVQ TYHVNMAGTN PYTTITPQEI 600
NGKWDHVRQL VPRRDQALTE EHSRQQHNER LRKQFGAQAN VIGPWIQTKM EEIGRISIEM 660
HGTLEDQLSH LRQYEKSIVN YKPKIDQLEG DHQLIQEALI FDNKHTNYTM EHIRVGWEQL 720
LTTIARTINE VENQILTRDA KGISQEQMNE FRASFNHFDR DHSGTLGPEE FKACLISLGY 780
DIGNDPQGEA EFARIMSIVD PNRLGVVTFQ AFIDFMSRET ADTDTADQVM ASFKILAGDK 840
NYITGDELRR ELPPDQAEYC IARMAPYAGP DSVPGALDYM SFSTALYGES DL 892 
Gene Ontology
 GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0043197; C:dendritic spine; IDA:UniProtKB.
 GO:0070062; C:extracellular vesicular exosome; IEA:Compara.
 GO:0005916; C:fascia adherens; IEA:Compara.
 GO:0005925; C:focal adhesion; IEA:Compara.
 GO:0005634; C:nucleus; IDA:RGD.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0001726; C:ruffle; IEA:UniProtKB-SubCell.
 GO:0030018; C:Z disc; IEA:UniProtKB-SubCell.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0051764; P:actin crosslink formation; IEA:InterPro.
 GO:0051017; P:actin filament bundle assembly; IEA:Compara.
 GO:0048041; P:focal adhesion assembly; IEA:Compara.
 GO:0051271; P:negative regulation of cellular component movement; IEA:Compara. 
Interpro
 IPR001589; Actinin_actin-bd_CS.
 IPR026921; Alpha-actinin.
 IPR001715; CH-domain.
 IPR011992; EF-hand-like_dom.
 IPR014837; EF-hand_Ca_insen.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom.
 IPR018159; Spectrin/alpha-actinin.
 IPR002017; Spectrin_repeat. 
Pfam
 PF00307; CH
 PF13405; EF_hand_4
 PF08726; efhand_Ca_insen
 PF00435; Spectrin 
SMART
 SM00033; CH
 SM00054; EFh
 SM00150; SPEC 
PROSITE
 PS00019; ACTININ_1
 PS00020; ACTININ_2
 PS50021; CH
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2 
PRINTS