UniProt Accession

 ARPC3_HUMAN; O15145; O00554 

Genbank Protein ID

 AF004561; AF006086; CR407667; BC067747; BC078162 

Genbank Nucleotide ID

 AAB61466.1; AAB64191.1; CAG28595.1; AAH67747.1; AAH78162.1 

Protein Name

 Actin-related protein 2/3 complex subunit 3 

Protein Synonyms/Alias

 Arp2/3 complex 21 kDa subunit; p21-ARC 

Gene Name

 ARPC3 

Gene Synonyms/Alias

 ARC21 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
14	SLMDPDTKLIGNMAL	ubiquitination	[1, 2]
29	LPIRSQFKGPAPRET	ubiquitination	[2, 3, 4]
37	GPAPRETKDTDIVDE	acetylation	[5]
37	GPAPRETKDTDIVDE	ubiquitination	[2, 3, 4]
50	DEAIYYFKANVFFKN	acetylation	[5]
56	FKANVFFKNYEIKNE	acetylation	[6]
56	FKANVFFKNYEIKNE	ubiquitination	[1]
61	FFKNYEIKNEADRTL	acetylation	[6]
61	FFKNYEIKNEADRTL	ubiquitination	[3]
93	NSKSQGEKEMYTLGI	ubiquitination	[4]
123	IYAKPANKQEDEVMR	ubiquitination	[2, 7]
147	TGLRLCEKVFDPQND	ubiquitination	[2, 7, 8]
155	VFDPQNDKPSKWWTC	acetylation	[7]
155	VFDPQNDKPSKWWTC	ubiquitination	[2, 7]
158	PQNDKPSKWWTCFVK	ubiquitination	[2, 7, 8]
165	KWWTCFVKRQFMNKS	ubiquitination	[1, 9]
171	VKRQFMNKSLSGPGQ	ubiquitination	[1, 2, 3, 8, 9, 10]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [6] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [7] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [8] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [9] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [10] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 

Functional Description

 Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. 

Sequence Annotation

 MOD_RES 47 47 Phosphotyrosine.
 MOD_RES 56 56 N6-acetyllysine.
 MOD_RES 61 61 N6-acetyllysine.  

Keyword

 Acetylation; Actin-binding; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 178 AA 

Protein Sequence

Gene Ontology

 GO:0005885; C:Arp2/3 protein complex; TAS:ProtInc.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0030027; C:lamellipodium; IEA:Compara.
 GO:0005200; F:structural constituent of cytoskeleton; TAS:ProtInc.
 GO:0034314; P:Arp2/3 complex-mediated actin nucleation; IEA:InterPro.
 GO:0006928; P:cellular component movement; TAS:ProtInc.
 GO:0038096; P:Fc-gamma receptor signaling pathway involved in phagocytosis; TAS:Reactome.
 GO:0045087; P:innate immune response; TAS:Reactome. 

Interpro

 IPR007204; ARP2/3_p21-Arc. 

Pfam

 PF04062; P21-Arc 

SMART

  

PROSITE

  

PRINTS