CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006154
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Centromere DNA-binding protein complex CBF3 subunit A 
Protein Synonyms/Alias
 Centromere-binding factor 2; Chromosome transmission fidelity protein 14; Kinetochore protein CTF14 
Gene Name
 CBF2 
Gene Synonyms/Alias
 CBF3A; CEP2; CTF14; NDC10; YGR140W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
556PDTHPTQKQESEGPLsumoylation[1]
651IRDNQPIKKEENIVNsumoylation[1]
779TGKFSTLKRPQLYMTsumoylation[1]
806KKLRTSGKYAETVKDacetylation[2]
Reference
 [1] Sumoylation of the budding yeast kinetochore protein Ndc10 is required for Ndc10 spindle localization and regulation of anaphase spindle elongation.
 Montpetit B, Hazbun TR, Fields S, Hieter P.
 J Cell Biol. 2006 Aug 28;174(5):653-63. [PMID: 16923829]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Acts as a component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromers and in several promoters. 
Sequence Annotation
 MOD_RES 566 566 Phosphoserine.
 MOD_RES 706 706 Phosphotyrosine.  
Keyword
 3D-structure; Centromere; Chromosome; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; DNA-binding; Nucleus; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 956 AA 
Protein Sequence
MRSSILFLLK LMKIMDVQQQ QEAMSSEDRF QELVDSLKPR TAHQYKTYYT KYIQWCQLNQ 60
IIPTPEDNSV NSVPYKDLPI SAELIHWFLL DTLITDDKPG EKREETEDLD EEEENSFKIA 120
TLKKIIGSLN FLSKLCKVHE NPNANIDTKY LESVTKLHTH WIDSQKAITT NETNNTNTQV 180
LCPPLLKVSL NLWNPETNHL SEKFFKTCSE KLRFLVDFQL RSYLNLSFEE RSKIRFGSLK 240
LGKRDRDAII YHKVTHSAEK KDTPGHHQLL ALLPQDCPFI CPQTTLAAYL YLRFYGIPSV 300
SKGDGFPNLN ADENGSLLQD IPILRGKSLT TYPREETFSN YYTTVFRYCH LPYKRREYFN 360
KCNLVYPTWD EDTFRTFFNE ENHGNWLEQP EAFAFPDKIP FDFKKIMNFK SPYTSYSTNA 420
KKDPFPPPKD LLVQIFPEID EYKRHDYEGL SQNSRDFLDL MEVLRERFLS NLPWIYKFFP 480
NHDIFQDPIF GNSDFQSYFN DKTIHSKGSP ILSFDILPGF NKIYKNKTNF YSLLIERPSQ 540
LTFASSHNPD THPTQKQESE GPLQMSQLDT TQLNELLKQQ SFEYVQFQTL SNFQILLSVF 600
NKIFEKLEMK KSSRGYILHQ LNLFKITLDE RIKKSKIDDA DKFIRDNQPI KKEENIVNED 660
GPNTSRRTKR PKQIRLLSIA DSSDESSTED SNVFKKDGES IEDGAYGENE DENDSEMQEQ 720
LKSMINELIN SKISTFLRDQ MDQFELKINA LLDKILEEKV TRIIEQKLGS HTGKFSTLKR 780
PQLYMTEEHN VGFDMEVPKK LRTSGKYAET VKDNDDHQAM STTASPSPEQ DQEAKSYTDE 840
QEFMLDKSID SIEGIILEWF TPNAKYANQC VHSMNKSGNK SWRANCEALY KERKSIVEFY 900
IYLVNHESLD RYKAVDICEK LRDQNEGSFS RLAKFLRKWR HDHQNSFDGL LVYLSN 956 
Gene Ontology
 GO:0031518; C:CBF3 complex; IDA:SGD.
 GO:0000778; C:condensed nuclear chromosome kinetochore; TAS:SGD.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
 GO:0051233; C:spindle midzone; IDA:SGD.
 GO:0005816; C:spindle pole body; IEA:UniProtKB-SubCell.
 GO:0019237; F:centromeric DNA binding; IDA:SGD.
 GO:0008301; F:DNA binding, bending; IDA:SGD.
 GO:0007059; P:chromosome segregation; IMP:SGD.
 GO:0000022; P:mitotic spindle elongation; IMP:SGD.
 GO:0000921; P:septin ring assembly; IMP:SGD. 
Interpro
 IPR022210; TF_OSM. 
Pfam
 PF12550; GCR1_C 
SMART
  
PROSITE
  
PRINTS