UniProt Accession

 D3Z2F2_MOUSE; D3Z2F2 

Genbank Protein ID

 AC108391 

Genbank Nucleotide ID

  

Protein Name

 60 kDa heat shock protein, mitochondrial 

Protein Synonyms/Alias

  

Gene Name

 Hspd1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
31	RAYAKDVKFGADARA	acetylation	[1, 2, 3]
31	RAYAKDVKFGADARA	succinylation	[2]
31	RAYAKDVKFGADARA	ubiquitination	[4]
58	VAVTMGPKGRTVIIE	ubiquitination	[4]
72	EQSWGSPKVTKDGVT	acetylation	[2, 3]
72	EQSWGSPKVTKDGVT	succinylation	[2]
72	EQSWGSPKVTKDGVT	ubiquitination	[4]
75	WGSPKVTKDGVTVAK	acetylation	[1, 2, 3]
75	WGSPKVTKDGVTVAK	succinylation	[2]
75	WGSPKVTKDGVTVAK	ubiquitination	[4]
82	KDGVTVAKSIDLKDK	acetylation	[2]
82	KDGVTVAKSIDLKDK	succinylation	[2]
82	KDGVTVAKSIDLKDK	ubiquitination	[4]
87	VAKSIDLKDKYKNIG	acetylation	[1, 3, 5]
89	KSIDLKDKYKNIGAK	acetylation	[5]
91	IDLKDKYKNIGAKLV	acetylation	[1, 3]
96	KYKNIGAKLVQDVAN	acetylation	[1, 3]
96	KYKNIGAKLVQDVAN	ubiquitination	[4]
125	VLARSIAKEGFEKIS	acetylation	[1, 2, 3, 5, 6, 7]
125	VLARSIAKEGFEKIS	succinylation	[2]
125	VLARSIAKEGFEKIS	ubiquitination	[4]
130	IAKEGFEKISKGANP	acetylation	[1, 2, 3, 5]
130	IAKEGFEKISKGANP	succinylation	[2]
133	EGFEKISKGANPVEI	acetylation	[1, 2, 3, 6, 7]
133	EGFEKISKGANPVEI	succinylation	[2]
133	EGFEKISKGANPVEI	ubiquitination	[4]
156	DAVIAELKKQSKPVT	acetylation	[1, 3, 5, 6, 7]
160	AELKKQSKPVTTPEE	acetylation	[3]
191	NIISDAMKKVGRKGV	acetylation	[1, 2, 3, 6, 8]
191	NIISDAMKKVGRKGV	succinylation	[2]
191	NIISDAMKKVGRKGV	ubiquitination	[4]

Reference

 [1] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [5] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [6] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [7] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [8] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 

Functional Description

  

Sequence Annotation

  

Keyword

 ATP-binding; Chaperone; Complete proteome; Cytoplasm; Nucleotide-binding; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 202 AA 

Protein Sequence

Gene Ontology

 GO:0009986; C:cell surface; IEA:Compara.
 GO:0005905; C:coated pit; IEA:Compara.
 GO:0030135; C:coated vesicle; IEA:Compara.
 GO:0019907; C:cyclin-dependent protein kinase activating kinase holoenzyme complex; IEA:Compara.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005769; C:early endosome; IEA:Compara.
 GO:0005615; C:extracellular space; IEA:Compara.
 GO:0046696; C:lipopolysaccharide receptor complex; IEA:Compara.
 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0005886; C:plasma membrane; IDA:MGI.
 GO:0030141; C:secretory granule; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0006919; P:activation of cysteine-type endopeptidase activity involved in apoptotic process; IEA:Compara.
 GO:0002368; P:B cell cytokine production; IEA:Compara.
 GO:0042100; P:B cell proliferation; IEA:Compara.
 GO:0048291; P:isotype switching to IgG isotypes; IEA:Compara.
 GO:0002755; P:MyD88-dependent toll-like receptor signaling pathway; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
 GO:0043065; P:positive regulation of apoptotic process; IEA:Compara.
 GO:0032727; P:positive regulation of interferon-alpha production; IDA:MGI.
 GO:0032729; P:positive regulation of interferon-gamma production; IDA:MGI.
 GO:0032733; P:positive regulation of interleukin-10 production; IEA:Compara.
 GO:0032735; P:positive regulation of interleukin-12 production; IEA:Compara.
 GO:0032755; P:positive regulation of interleukin-6 production; IEA:Compara.
 GO:0043032; P:positive regulation of macrophage activation; IEA:Compara.
 GO:0002842; P:positive regulation of T cell mediated immune response to tumor cell; IEA:Compara.
 GO:0042026; P:protein refolding; IEA:Compara.
 GO:0006986; P:response to unfolded protein; IEA:Compara.
 GO:0042110; P:T cell activation; IDA:MGI. 

Interpro

 IPR017998; Chaperone_TCP-1.
 IPR002423; Cpn60/TCP-1.
 IPR027413; GROEL-like_equatorial. 

Pfam

 PF00118; Cpn60_TCP1 

SMART

  

PROSITE

  

PRINTS

 PR00304; TCOMPLEXTCP1.