UniProt Accession

 SKP1_YEAST; P52286; D6VSW0; Q07186 

Genbank Protein ID

 U43179; U61764; U32517; AY557730; BK006938 

Genbank Nucleotide ID

 AAB17500.1; AAC49492.1; AAB64763.1; AAS56056.1; DAA12170.1 

Protein Name

 Suppressor of kinetochore protein 1 

Protein Synonyms/Alias

 Centromere DNA-binding protein complex CBF3 subunit D; E3 ubiquitin ligase complex SCF subunit SKP1 

Gene Name

 SKP1 

Gene Synonyms/Alias

 CBF3D; YDR328C; D9798.14 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
60	SETNHKSKDNNNGDD	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Essential component of the E3 ubiquitin ligase complex SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins like phosphorylated SIC1. Participates in the attachment of chromosomes to the spindle. Acts as a regulatory component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromeres and in several promoters. The association of CBF3C with CBF3D and SGT1 is required for CBF3C activation and CBF3 assembly. SKP1/CBF3D could retrieve cyclins or cyclin-CDK- like proteins into the kinetochore thus providing cell cycle- regulated kinetochore activity. Involved in the regulation of methionine biosynthesis genes. Facilitates association of CDC53 with CDC4 and of ROY1 with YPT52. 

Sequence Annotation

 REGION 135 194 Interaction with the F-box domain of F-
 MOD_RES 177 177 Phosphothreonine.  

Keyword

 3D-structure; Centromere; Chromosome; Complete proteome; Cytoplasm; Direct protein sequencing; DNA-binding; Kinetochore; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 194 AA 

Protein Sequence

Gene Ontology

 GO:0031518; C:CBF3 complex; IDA:SGD.
 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0000776; C:kinetochore; TAS:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0043291; C:RAVE complex; IPI:SGD.
 GO:0019005; C:SCF ubiquitin ligase complex; IDA:SGD.
 GO:0003688; F:DNA replication origin binding; IPI:SGD.
 GO:0000910; P:cytokinesis; IMP:SGD.
 GO:0000082; P:G1/S transition of mitotic cell cycle; IMP:SGD.
 GO:0000086; P:G2/M transition of mitotic cell cycle; IMP:SGD.
 GO:0051382; P:kinetochore assembly; IDA:SGD.
 GO:0045116; P:protein neddylation; IMP:SGD.
 GO:0042787; P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process; IDA:SGD.
 GO:0007096; P:regulation of exit from mitosis; IMP:SGD.
 GO:0043254; P:regulation of protein complex assembly; IPI:SGD.
 GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IDA:SGD.
 GO:0000921; P:septin ring assembly; IMP:SGD.
 GO:0007035; P:vacuolar acidification; IMP:SGD. 

Interpro

 IPR011333; BTB/POZ_fold.
 IPR016897; E3_ubiquit_lig_SCF_Skp.
 IPR001232; Skp1_comp.
 IPR016072; Skp1_comp_dimer.
 IPR016073; Skp1_comp_POZ. 

Pfam

 PF01466; Skp1
 PF03931; Skp1_POZ 

SMART

 SM00512; Skp1 

PROSITE

  

PRINTS