CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003556
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Exopolyphosphatase 
Protein Synonyms/Alias
 ExopolyPase; Metaphosphatase 
Gene Name
 ppx 
Gene Synonyms/Alias
 b2502; JW2487 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
103LNATDFLKRAEKVIPacetylation[1]
107DFLKRAEKVIPYPIEacetylation[1]
136VEHTQPEKGRKLVIDacetylation[1]
184FPGGVINKENFQRARacetylation[1]
197ARMAAAQKLETLTWQacetylation[1]
233VLMEMGEKDGIITPEacetylation[1]
244ITPERLEKLVKEVLRacetylation[1]
353QWREQQPKLAHPQLEacetylation[1]
414TLVRYHRKAIKLDDLacetylation[1]
417RYHRKAIKLDDLPRFacetylation[1]
428LPRFTLFKKKQFLPLacetylation[1]
429PRFTLFKKKQFLPLIacetylation[1]
430RFTLFKKKQFLPLIQacetylation[1, 2]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111] 
Functional Description
 Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate. 
Sequence Annotation
  
Keyword
 3D-structure; Cell membrane; Complete proteome; Direct protein sequencing; Hydrolase; Magnesium; Membrane; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 513 AA 
Protein Sequence
MPIHDKSPRP QEFAAVDLGS NSFHMVIARV VDGAMQIIGR LKQRVHLADG LGPDNMLSEE 60
AMTRGLNCLS LFAERLQGFS PASVCIVGTH TLRQALNATD FLKRAEKVIP YPIEIISGNE 120
EARLIFMGVE HTQPEKGRKL VIDIGGGSTE LVIGENFEPI LVESRRMGCV SFAQLYFPGG 180
VINKENFQRA RMAAAQKLET LTWQFRIQGW NVAMGASGTI KAAHEVLMEM GEKDGIITPE 240
RLEKLVKEVL RHRNFASLSL PGLSEERKTV FVPGLAILCG VFDALAIREL RLSDGALREG 300
VLYEMEGRFR HQDVRSRTAS SLANQYHIDS EQARRVLDTT MQMYEQWREQ QPKLAHPQLE 360
ALLRWAAMLH EVGLNINHSG LHRHSAYILQ NSDLPGFNQE QQLMMATLVR YHRKAIKLDD 420
LPRFTLFKKK QFLPLIQLLR LGVLLNNQRQ ATTTPPTLTL ITDDSHWTLR FPHDWFSQNA 480
LVLLDLEKEQ EYWEGVAGWR LKIEEESTPE IAA 513 
Gene Ontology
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0004309; F:exopolyphosphatase activity; IDA:EcoCyc.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0006798; P:polyphosphate catabolic process; IMP:EcoCyc. 
Interpro
 IPR022371; Exopolyphosphatase.
 IPR003695; Ppx_GppA. 
Pfam
 PF02541; Ppx-GppA 
SMART
  
PROSITE
  
PRINTS